黄精多糖对力竭小鼠脑组织自由基代谢影响  

Effects of polygonatum sibiricum polysaccharides on free radical metabolism in brain tissue of mice at exhaustion and recovery

作　　者：王玉勤 于晓婷 吴晓岚 王昊霖[2] 张广新 崔培红 

机构地区：[1]辽宁卫生职业技术学院,辽宁沈阳110101 [2]沈阳市第四人民医院

出　　处：《中国公共卫生》2014年第9期1165-1167,共3页Chinese Journal of Public Health

基　　金：辽宁卫生职业技术学院科学研究基金(2012Y06)

摘　　要：目的观察黄精多糖对力竭小鼠脑组织自由基代谢的影响,探讨其抗疲劳的机制。方法昆明种小鼠72只,随机分为对照组和黄精多糖低、高剂量组。经口灌胃4周,将3组小鼠分别于安静时、力竭即刻、力竭恢复24 h3种状态下断头处死,测定脑组织中丙二醛(MDA)含量,超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)的活性。结果力竭即刻及力竭恢复24 h,高剂量组MDA含量分别为(3.46±0.29)、(2.29±0.55)nmol/mg,明显低于对照组和低剂量组(P<0.01);高剂量组SOD活性分别为(100.64±10.12)、(114.58±9.50)U/mg,GSH-Px活性分别为(2.17±0.35)、(2.45±0.27)U/mg,与对照组相比差异有统计学意义(P<0.01);T-AOC活性分别为(0.50±0.09)、(0.75±0.09)U/mg,明显高于对照组和低剂量组(P<0.01)。结论黄精多糖通过增强小鼠脑组织抗氧化能力,发挥抗疲劳作用。Objective To observe the effects of polygonatum sibiricum polysaccharides （PSP） on free radical metabolism in brain tissue of the mice at the time of exhaustion and recovery. Methods Seventy-two Kunming mice were randomly divided into three groups ：normal group（N） ,high （H） and low（L） dose PSP group. The mice in the H and L dose groups were administered with PSP by gavage once a day for 4 weeks and those in the N group were treated with a same amount of normal saline. By the end of the treatment, the mice of the 3 groups were sacrificed at the time of rest state, the immediate end of exhaustive exercise and 24 hours after the end of exhaustive exercise, respectively. The content of malonaldehyde（ MDA）, the activity of superoxide dismutase （SOD） and glutathione peroxidase （GSH-Px）, and total antioxidant capacity （T-AOC）in brain tissue of the mice were measured. Results For the mice in H group, the contents of MDA （3.46 ±0. 29 at the immediate end of exhaustive exercise,2. 29±0. 55 nmol/mg 24 hours after the end of ex- haustive exercise）were significantly lower than those of the mice in N and L group （P 〈 0.01, P 〈 0.05 ）, and the activi- ties of SOD （ 100. 64 ± 10. 12,114. 58 ± 9. 50 U/mg）, GSH-Px （2. 17 ± 0. 35,2.45 ± 0. 27 U/mg）, and T-AOC （0. 50 ＋_ 0.09,0. 75±0. 09 U/mg ） were all higher than those of the mice in N and L group. Conclusion PSP can improve an- tioxidant capacity of brain tissue and has significant anti-fatigue function in mice.

关 键 词：黄精多糖 脑组织 自由基 抗氧化 

分 类 号：R338.2[医药卫生—人体生理学]