N/P比及氮盐浓度对眼点拟微绿球藻培养密度和油脂含量的影响  

EFFECT OF N/P RATIOS AND NITROGEN CONCENTRATION ON LIPID CONTENT AND GROWTH DENSITY OF Nannochloropsis oculata

作　　者：窦晓[1] 陆向红[1] 卢美贞[1] 薛蓉[1] 晏荣军[1] 计建炳[1] 

机构地区：[1]浙江工业大学化学工程与材料学院,浙江省生物质燃料利用技术研究重点实验室,杭州310014

出　　处：《太阳能学报》2014年第8期1536-1540,共5页Acta Energiae Solaris Sinica

基　　金：浙江省重大科技专项(2006C11015);浙江省重点科技创新团队(2009R50012-11);浙江省新苗人才计划(Y201224695);浙江工业大学大学生课外科技基金

摘　　要：研究N/P比及Na NO3浓度对眼点拟微绿球藻生长密度和油脂含量的影响。设计4个不同N/P比(=1∶1,4∶1,16∶1,32∶1)和6个不同Na NO3浓度(0.025、0.075、0.225、0.450、0.900、1.350 g/L)。实验结果表明:N/P比及Na NO3浓度均对眼点拟微绿球藻的生长密度和油脂含量有很大影响。当Na NO3浓度为0.225 g/L,N/P比为4∶1时,其油脂含量和生物量最高,分别为41.20%和2.53 g/L。实验找到眼点拟微绿球藻高油脂含量和高生长密度的N/P比和Na NO3浓度的最佳结合点,为眼点拟微绿球藻能够用来生产生物柴油提供实验依据。The effects of N/P ratios and nitrogen concentration on the lipid content and growth density of N. oculata were studied. The N. oculata was cultivated under four different N/P ratios （N/P ratios=1： 1, 4： 1, 16： 1, 64： 1） and six different nitrogen concentrations level （0.025, 0.075, 0.225, 0.450, 0.900, 1.350 g/L）. It was found that both of the lipid content and growth density was strongly influenced by the variation of test parameters. The lipid content grew to 41.20% and the growth density increased to 2.53 g/L when nitrogen concentration in the medium was 0.225 g/L, N/P ratio was 4： 1. The optimized balance between high lipid content and growing density of N. oculata was found by adjusting N/P ratio and nitrogen concentration, which would provide the experimental basis that N. oculata could be used to produce bio-diesel.

关 键 词：生物柴油 眼点拟微绿球藻 N P比 氮盐浓度 

分 类 号：TE669[石油与天然气工程—油气加工工程]