胃癌N糖特征性改变及其与中医证型的相关性研究  

A Relevant Research on Changes in Characteristics of N-glycan in Gastric Cancer and Its Relationship with TCM Syndromes

作　　者：张卫星[1] 阮奕[2] 顾群浩[3] 矫健鹏[4] 刘龙[2] 

机构地区：[1]丽水市中医院,浙江丽水323000 [2]第二军医大学附属长海医院,上海200433 [3]上海中医药大学附属岳阳中西医结合医院,上海200437 [4]上海长征医院,上海200006

出　　处：《中国中医药信息杂志》2014年第9期36-40,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81102693;81102565);上海市科委中药现代化专项(09dZ1970800)

摘　　要：目的探讨胃癌N糖的特征性改变及其与证型的相关性。方法采集138例胃癌患者和128例健康人的静脉血,采用基于DNA测序仪的荧光糖电泳法检测血中N糖变化,比较不同证型患者的N糖差异。结果所有标本均可得到9个基本N糖糖峰,胃癌患者N糖糖峰Peak1、Peak5、Peak9和Peak2均显著升高(P<0.05,P<0.01),Peak3、Peak6显著降低(P<0.01)。Ⅰ期胃癌的Peak6峰值显著高于Ⅱ、Ⅲ、Ⅳ期胃癌(P<0.01),Peak9峰值显著低于Ⅱ、Ⅲ、Ⅳ期胃癌(P<0.01)。肝胃不和型Peak1峰值显著低于痰湿凝结型、瘀毒内阻型和气血双亏型(P<0.05,P<0.01),胃热伤阴型、脾胃虚寒型Peak1峰值显著低于气血双亏型(P<0.01),痰湿凝结型、瘀毒内阻型Peak1峰值显著低于气血双亏型(P<0.05);肝胃不和型Peak6峰值显著高于胃热伤阴型、痰湿凝结型、瘀毒内阻型和气血双亏型(P<0.01);气血双亏型Peak9峰值显著高于肝胃不和型、胃热伤阴型和脾胃虚寒型(P<0.05,P<0.01)。结论胃癌外周血中N糖存在特征性改变,N糖的特征性改变促进了胃癌侵袭转移,与胃癌证型有一定相关性。Objective To discuss the changes in characteristics of N-glycan in gastric cancer and its relationship with TCM syndromes. Methods The blood samples of 138 gastric cancer patients and 120 healthy volunteers were collected. The changes in N-glycan were detected by DNA sequencer-assisted and fluorophore-assisted carbohydrate electrophoresis （DSA-FACE）, and differences of N-glycan among different TCM syndromes were compared. Results At least 9 N-glycan peaks could be identified in all samples. Compared with the healthy volunteers, Peakl, Peak5, Peak9 and Peak2 of gastric cancer patients obviously increased （P〈0.05, P〈0.01）, whereas Peak3, Peak6 significantly decreased （P〈0.01）. Peak6 of gastric cancer in stage I was obviously higher than stages If, III, and IV （P〈0.01）, while Peak9 in stage I was obviously lower than the other three stages （P〈0.01）. Peak1 was significantly lower in disharmony between liver and stomach type than stagnation of phlegm-dampness type, interior retention of toxin stagnation type, deficiency of both Qi and blood type （P〈0.05, P〈0.01）; lower in impairment ofyin due to stomach heat type, deficiency-cold in spleen blood type （P〈0.01）; lower in stagnation of stagnation type than deficiency of both Qi and stomach type than deficiency of both Qi and phlegm-dampness type, interior retention of toxin and blood type （P〈0.05）. Peak6 was higher in disharmony between liver and stomach type than impairment of yin due to stomach heat type, stagnation of phlegm-dampness type, interior retention of toxin stagnation type than deficiency of both Qi and blood type （P〈0.01）. Peak9 was much higher in deficiency of both Qi and blood type than disharmony between liver and stomach type （P〈0.01）, impairment of yin due to stomach heat type and deficiency-cold in spleen and stomach type （Pdisharmony between liver and stomach type than impairment of yin due to stomach heat type, stagnation of phlegm-dampness type, interior retention of toxin stagnation t

关 键 词：N糖 胃癌 中医证型 

分 类 号：R259.73[医药卫生—中西医结合]