愈肾合剂对糖尿病模型大鼠肾组织血红素氧合酶-1和血红素氧合酶-2表达的影响  

Effects of Yushen Heji on Expressions of Heme Oxygenase-1 and Heme Oxygenase-2 in Kidney of Diabetic Rats

作　　者：曹焕敏[1] 成秀梅[2] 王晓松[2] 王蓓[2] 

机构地区：[1]石家庄市中医院,河北石家庄050051 [2]河北中医学院中西医结合学院,河北石家庄050091

出　　处：《中国中医药信息杂志》2014年第9期61-63,共3页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：河北省教育厅科研计划(2007439)

摘　　要：目的观察愈肾合剂对糖尿病模型大鼠肾脏组织血红素氧合酶(HO)-1、HO-2的影响,探讨其治疗糖尿病肾病作用机制。方法 SD大鼠随机分为正常组、模型组、对照组、治疗组。采用皮下注射链脲佐菌素法复制模型,对照组和治疗组给予相应药物灌胃,正常组和模型组灌胃等量蒸馏水。给药8周后,收集尿液测定24 h尿蛋白(Upro)、尿肌酐(Ucr),断头取血测定一氧化碳(CO)、尿素氮(BUN)、血肌酐(Scr),采用免疫组织化学法检测肾脏组织HO-1、HO-2的表达。结果与正常组比较,模型组大鼠BUN、24 h Upro、Scr升高,肌酐清除值(Ccr)、血浆CO降低,肾脏组织的HO-1、HO-2表达明显减弱(P<0.01);与模型组比较,治疗组和对照组BUN、24 h Upro、Scr明显下降,Ccr、血浆CO活性升高,肾脏组织的HO-1、HO-2表达明显增强(P<0.05,P<0.01);与对照组比较,治疗组24 h Upro降低,血浆CO活性升高,肾脏组织HO-1、HO-2表达明显增强(P<0.05,P<0.01)。结论愈肾合剂对糖尿病肾病大鼠有一定的治疗作用,可能是通过HO/CO系统实现的。Objective To observe the effects of Yushen Heji on expressions of Heme Oxygenase （HO-1） and HO-2 in kidney of diabetic rats： To explore its mechanism in diabetic nephropathy. Methods SD rats were randomly divided into normal group, model group, control group and treatment group. Models were duplicated by subcutaneous injection STZ. Control group and treatment group were given gavage with related medicine, while normal group and model group were given gavage with the same amount of distilled water. Eight weeks later, the urine of rats was collected to detect the 24 h Upro and Ucr： the rats were sacrificed to detect the levels of CO, BUN and Scr： the kidneys were harvested for observing the expressions of HO-1 and HO-2 by immunohistochemistry. Results Compared with normal group, the contents of BUN, 24 h Upro and Scr all increased in model group; Ccr and plasma CO decreased;the expressions of HO-1 and HO-2 in kidney tissues were obviously reduced （P 〈 0.01）. Compared with model group, the contents of BUN, 24 h Upro and Scr all obviously decreased in treatment group and control group; Ccr and plasma CO increased： the expressions of HO- 1 and HO-2 in kidney tissues obviously increased （P〈0.05, P〈0.01）. Compared with control group, the contents of 24 h Upro decreased in treatment group; plasma CO increased; the expressions of HO-1 and HO-2 in kidney tissues obviously increased （P〈0.05, P〈0.01）. Conclusion Yushen Heji has therapeutic effect on diabetic nephropathy rats, which may related with HO/CO system.

关 键 词：愈肾合剂 糖尿病肾病 内源性一氧化碳 血红素氧合酶-1 血红素氧合酶-2 大鼠 

分 类 号：R285.5[医药卫生—中药学]