双黄连注射液类过敏反应研究  

Study on Anaphylactoid Reaction Induced by Shuanghuanglian Injection

作　　者：黄崇刚[1] 莫宗成[1] 吴思澜 罗超利[1] 王敏[1] 

机构地区：[1]重庆市中药研究院,重庆400065

出　　处：《中国中医药信息杂志》2014年第9期64-67,共4页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：重庆市卫生局中医药科技项目(2011-2-118);重庆市科委项目(cstc2012pt-kyys10001)

摘　　要：目的观察双黄连注射液的类过敏反应,测定致敏动物血清组胺含量,探讨其与类过敏反应的相关性。方法将豚鼠随机分组,选用双黄连注射液按不同注射速度、不同剂量进行给药,观察豚鼠的类过敏反应,同时用ELISA进行血清免疫球蛋白E(IgE)、血清组胺含量检测。结果不同剂量、不同注射速度下的双黄连注射液均能引起豚鼠的类过敏症状;不同剂量、不同注射速度下的双黄连注射液组IgE水平与生理盐水组无明显差异;不同剂量、不同注射速度下的双黄连注射液组血清组胺水平明显升高,且呈剂量、速度相关性。结论双黄连注射液能引起豚鼠产生类过敏反应,豚鼠血清组胺含量的变化与双黄连注射液的给予剂量、速度有相关性。Objective To observe the anaphylactoid reactions of Shuanghuanglian injection, determine the serum histamine level of sensitized animal, and to investigate its correlation with anaphylactoid reactions. Methods The guinea pigs were randomly selected to observe the anaphylactoid reactions using Shuanghuanglian injections in the different injection speed or different doses, and the serum histamine and the serum IgE of sensitized animals were determined by ELISA. Results The anaphylactoid reactions were induced by Shuanghuanglian injections in the different conditions of injection speed and doses in guinea pigs. There were no obvious difference about the serum IgE level between Shuanghuanglian group and saline group were injected in injection speed and doses. The serum histamine level was significantly increased in Shuanghuanglian group both different conditions of injection speed and doses, and it related to doses and speed. Conclusion Shuanghuanglian injection can cause anaphylactoid reactions in guinea pigs, and the changes of serum histamine level are related to injection speed and doses of Shuanghuanglian injection.

关 键 词：双黄连注射液 类过敏反应 血清免疫球蛋白E 血清组胺 

分 类 号：R285.6[医药卫生—中药学]