3个不同地理日本沼虾天然群体遗传多样性的SSR分析  

Genetic Diversity in Three Different Natural Populations of Macrobrachium nipponense Revealed by SSR Markers

作　　者：夏建海[1] 范武江[2] 王晓清[1] 马晓[1] 戴振炎[1] 王璐明[1] 

机构地区：[1]湖南农业大学动物科技学院,中国长沙410128 [2]上海市水产研究所,中国上海200433

出　　处：《湖南师范大学自然科学学报》2014年第4期23-28,共6页Journal of Natural Science of Hunan Normal University

基　　金：上海市农业委员会资助项目(沪农科推字(2011)第6-1号)

摘　　要：利用11对微卫星引物对洞庭湖、鄱阳湖、龙感湖的日本沼虾天然群体进行遗传多样性的分析，评价不同地理日本沼虾天然群体的遗传多样性和遗传分化水平．在3个日本沼虾群体中共获得90个等位基因，各位点平均等位基因为8．182个，各群体的平均等位基因数在（A）32～38之间，平均多态信息含量（PIC）介于0．5907～0．6430，平均期望杂合度He 为0．6577～0．7055、平均观测杂合度Ho为0．5141～0．5799．群体间各位点Shannon指数均大于1，Fis均为正值，群体间平均近交系数为19％，Fst值显示整个群体分化程度较低，其中遗传变异的5.38％存在于群体之间，94．62％存在于个体之间．结果表明：3个日本沼虾天然群体的遗传多样性较高，分化程度较低，其中鄱阳湖群体遗传多样性最丰富，遗传分化水平最低．To evaluate the genetic diversity and genetic differentiation of the different natural populations of Macrobrachium nipponense, 11 microsatellite locus were used to analyze the genetic diversity in three natural popu-lations of Macrobrachium nipponense Dongting , Poyang and Longgan .The results showed that 11 microsatellite locus were highly polymorphic .A total of 90 alleles were detected, each loci had 8.182 alleles.In these natural popula-tions the average number of allele （ A ） ranged from 32 to 38 , and the value of average polymorphism information content （ PIC ） was 0.590 7 to 0.643 0, the value of average expected heterozygosity He was 0.657 7 to 0.705 5, the value of average observed heterozygosity Ho in three populations ranged from 0.514 1 to 0.579 9.Shannon in-dexes were greater than 1.Fis were positive, average inbreeding coefficient of populations was 19%.Fst Values show that the whole populational differentiation degree was lower .Most variation occurred within populations （94.62） among population （5.38）.The results showed that the genetic diversity is richer in three natural popula-tions of Macrobrachium nipponense, and Poyang is the most abundant in genetic diversity population and the lowest level of genetic differentiation .

关 键 词：日本沼虾 微卫星 遗传多样性 遗传分化 

分 类 号：S917[农业科学—水产科学]