基于碳纳米管的高灵敏度免标记电化学核酸适体传感电极  

A Sensitive and Label-Free Electrochemical Aptasensor based on Signal Amplification of Carbon Nanotubes

作　　者：邓春艳[1,2] 范慧敏[2] 向娟[2] 李元建[1] 

机构地区：[1]中南大学药学院,湖南长沙410083 [2]中南大学化学化工学院,湖南长沙410083

出　　处：《电化学》2014年第4期386-391,共6页Journal of Electrochemistry

基　　金：国家自然科学基金项目(No.21005090);中国博士后基金项目(No.2012M510136);中国博士基金特别资助项目(No.2013T60774)资助

摘　　要：以电活性钌化合物[Ru（NH3）6]3＋为信号传感源,借助碳纳米管构建了高灵敏检测腺苷免标记电化学传感电极（BSA/Apt/CNTs/GC）.BSA/Apt/CNTs/GC电极在最佳实验条件下检测腺苷线性范围为5.0×10-11~1.0×10-7mol·L-1,检测下限为2.7×10-11mol·L-1.该传感电极有较高的灵敏度、良好的选择性、重现性和稳定性.与传统标记型适体传感电极相比,其制作简便,也许还适用于其他小分子和蛋白质的检测,有一定的普适性.A label-free electrochemical sensing electrode for highly sensitive detection of adenosine was constructed based on the signal amplification of carbon nanotubes （CNTs）. The change in the interfacial feature of the modified electrode was characterized by electrochemical impedance spectroscopy. Using [Ru0WH3）6]3＋ as the signaling moiety, adenosine with concentrations as low as 0.027 nmol -L-1 can be selectively detected. Additionally, the fabrication of this present aptasensor was simple, time-saving and cost-effective. Compared with other reported aptasensors, the proposed aptasensorhad advantages of excellent sensitivity, selectivity and simplicity, which plays a potential role in development of aptasensor.

关 键 词：核酸适体 腺苷 免标记 碳纳米管 

分 类 号：O646.54[理学—物理化学] O657.1[理学—化学]