外源性H2S对肺移植术大鼠肺缺血再灌注时一氧化氮／一氧化氮合酶的影响  

Effects of exogenous hydrogen sulfide on nitric oxide and nitric oxide synthase during lung ischemia- reperfusion in a rat model of lung transplantation

作　　者：朱宏伟[1] 吴镜湘[1] 陈旭[1] 徐美英[1] 

机构地区：[1]上海交通大学附属胸科医院麻醉科,上海200030

出　　处：《中华麻醉学杂志》2014年第6期727-729,共3页Chinese Journal of Anesthesiology

基　　金：上海市卫生局科研项目（20124331）;上海市胸科医院院级课题（YZl3-33）

摘　　要：目的评价外源性H2S对肺移植术大鼠肺缺血再灌注时一氧化氮（N0）／一氧化氮合酶的影响。方法清洁级雄性SD大鼠40只，体重250～350g，2-3月龄，采用随机数字表法，将其分为4组（n=10）：假手术组（S组）、肺移植术组（L组）、肺移植术＋H2S生成酶胱硫醚．γ-裂解酶抑制剂炔丙基甘氨酸组（PPG组）和肺移植术＋外源性H2S供体NariS组（NaHS组）。采用改良三袖套法制备大鼠左肺原位移植术模型，冷缺血时间为50～70min；PPG组和NaHS组分别于移植肺肺门开放前5min腹腔注射炔丙基甘氨酸37．5mg／kg和NaHS14μmol／kg。再灌注2h时取左肺组织，测定肺组织湿／干重（W／D）比值、丙二醛（MDA）、髓过氧化物酶（MPO）、诱导型一氧化氮合酶（iNOS）、内皮型一氧化氮合酶（eNOS）和NO的水平；光镜下观察肺组织病理学结果。结果与S组比较，L组肺组织W／D比值、MDA、iNOS、NO和MPO的水平升高，肺组织eNOS含量降低（P〈0．05），NaHS组上述指标差异无统计学意义（P〉0．05）；与L组比较，PPG组肺组织W／D比值、MDA、iNOS、NO和MPO的水平升高，肺组织eNOS含量降低，NaHS组肺组织W／D比值、MDA、iNOS、NO和MPO的水平降低，肺组织eNOS含量升高（P〈0．05）。NaHS组肺组织病理学损伤明显轻于L组。结论外源性Hs可减轻肺移植术大鼠肺缺血再灌注损伤，其机制与降低iNOS活性和升高eNOS活性有关。Objective To evaluate the effects of exogenous hydrogen sulfide （H2S） on nitric oxide （NO） and nitric oxide synthase （NOS） during lung ischemia-reperfugion in a rat model of lung transplantation. Methods Forty pathogen-free male Sprague-Dawley rats, weighing 250-350 g, aged 2-3 months, were used as donor and recipient rats in this study. The animals were randomly divided into 4 groups （ n = 10 each） ： sham operation group （group S）; lung transplantation group （group L）; lung transplantation ＋ H2S synthesis-CSE inhibitor D, L- propargylgylcine （PPG） group （group PPG）; lung transplantation ＋ exogenous H2S donor NariS group （group NariS） . The donor lungs were removed from live donor rats and placed in low potassium dextran solution at 4 ℃ . Orthotopic left lung transplantation was performed using modified three-cuff technique and the time for cold ischemia was 50-70 min. In PPG and Naris groups, PPG 37.5 mg/kg and Naris 14 /~mol/kg were injected intraperitoneally at 5 min before opening of the hilum of the transplanted lung. The ：implanted donor lungs were ventilated and reperfused. The donor lungs were removed after being implanted, ventilated and reperfused for 2 h for microscopic examination and for determination of wet to dry lung weight （W/D） ratio and the levels of malondialdehyde （MDA）, myeloperoxidase （MPO）, inducible NOS （iNOS）, endothelial NOS （eNOS）, and NO. Results Compared with group S, W/D ratio and the levels of MDA, iNOS, NO and ＂MPO were significantly increased, and the content of eNOS was decreased in group L, and no significant change was found in the parameters mentioned above in group NariS. Compared with group L, W/D ratio and the levels of MDA, iNOS, NO and MPO were significantly increased, and the content of eNOS was decreased in group PPG, and W/D ratio and the levels of MDA, iNOS, NO and MPO were decreased, and the content of eNOS was increased in group NariS. The pathological changes of lungs were significantly attenuate

关 键 词：硫化氢 肺移植 再灌注损伤 一氧化氮 一氧化氮合酶 

分 类 号：R655.3[医药卫生—外科学]