类风湿关节炎患者血浆Ghrelin水平变化及其基因C408A多态性分析  

Ghrelin plasma level changes and Ghrelin gene C408A polymorphism in patients with rheumatoid arthritis

作　　者：杨梅花[1] 王向红[2] 冯忠军[3] 米娇[1] 张志红[1] 

机构地区：[1]石家庄市第一医院,石家庄050000 [2]石家庄市第六医院 [3]河北医科大学第三医院

出　　处：《山东医药》2014年第31期21-23,共3页Shandong Medical Journal

基　　金：河北省科技计划项目(132777172)

摘　　要：目的观察类风湿关节炎(RA)患者血浆Ghrelin水平改变及其基因C408A的多态性,进一步探讨RA发病机制。方法采集120例RA患者(RA组)与150例健康查体者(对照组)的静脉血,采用ELISA法测定血浆Ghrelin水平,聚合酶链反应—限制性片段长度多态性(PCR-RFLP)法检测Ghrelin基因C408A的多态性,分析基因型与RA患者血浆Ghrelin水平、病程、病情的关系。结果 RA组和对照组血浆Ghrelin分别为(115.03±38.12)、(156.28±65.84)pg/mL,组间比较,P<0.05;两组基因型和等位基因频率比较均有显著差异(P均<0.05);RA组基因型为CC、CA、AA者血浆Ghrelin水平、病程比较均有显著差异(P均<0.05),但病情无显著差异(P>0.05)。结论 RA患者存在血浆Ghrelin水平降低及Ghrelin基因C408A位点变异,纯合的AA型和杂合的CA型均可比野生的CC型增加RA患病风险,但Ghrelin基因可能并非河北汉族RA的主效基因。Objective To observe the Ghrelin plasma level changes and its gene C 408A polymorphism in patients with rheumatoid arthritis （ RA） and to further investigate the pathogenesis .Methods We collected the vein blood from 120 ca-ses of RA patients （ RA group） and 150 cases of healthy people （ control group ） .The plasma level of Ghrelin was deter-mined by ELISA, and the single nucleotide polymorphisms （ SNPs） of Ghrelin gene C408A were detected by PCR-RFLP method.The relationships of genotypes with the Ghrelin plasma level , course of disease, state of disease in patients with RA were analyzed.Results The plasma levels of Ghrelin in the RA group and control group were （115.03 ±38.12） and （156.28 ±65.84） pg/mL, respectively （P〈0.05）; significant differences were found in the genotypes and fre-quencies between the two group （all P〈0.05）.The plasma level of Ghrelin and course of disease were statistically differ-ent in the genotypes of CC, CA and AA in the RA group （all P〈0.05）, but no significant difference was found in the state of disease （P〉0.05）.Conclusions In RA patients, the plasma level of Ghrelin is decreased , and there is Ghrelin gene C408A mutation.Both the homozygous AA and heterozygous CA can increase the risk of RA than the wild genotype CC, but the Ghrelin gene may be not the main gene in Hebei RA patients of the Han nationality .

关 键 词：类风湿关节炎 GHRELIN基因 单核苷酸多态性 

分 类 号：R593.22[医药卫生—内科学]