锌指核酸酶及其应用研究进展  

Research Development on Zinc Finger Nucleases and Its Application

作　　者：李万利[1,2] 李文清[1,3] 王明发[2] 王二耀[2] 曹文广[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]河南省农业科学院畜牧兽医研究所,河南郑州450002 [3]河南农业大学生命科学学院,河南郑州450002

出　　处：《河南农业科学》2014年第8期1-9,共9页Journal of Henan Agricultural Sciences

基　　金：国家博士后科学基金项目(2011M500461);河南省财政预算项目(20137918)

摘　　要：锌指核酸酶由锌指和非限制性切割酶两部分组成,锌指能特异性结合基因组双链DNA,非限制性切割酶能造成染色体上双链DNA的断裂,通过机体的修复机制可以使目的基因突变或外源基因插入,进而应用于转基因动物的制备。综述了锌指、切割酶及采用锌指核酸酶制备转基因动物的研究进展,主要包括锌指的发现、结构及与DNA结合时的碱基分布和结合方式,锌指的筛选和设计方法;切割酶的研究进展包括FokⅠ的发现及切割特点,基因修饰后的异源二聚体切割特异性等。Zinc finger nuclease was made of zinc finger and nonspecific cleavage nuclease.Zinc fingers could bind the double-stranded DNA specially.Non-specific cleavage nucleases could make double-stranded DNA break in chromosomal.The target gene mutation such as gene mutation or exogenous gene insertion happened by repairing mechanismin vivo.It could be used in transgentic animal production normally.The development of the research on zinc finger,cleavage enzyme and production of transgentic animal using zinc finger nucleases was summarized in this review.The discovery and the structure of zinc finger,its binding manners with DNA,selecting and designing methods were first discussed.Then the discovery of cleavage enzyme,charactersistics of FokⅠ on cutting the DNA double strains,and specialities of engineered cleavage enzyme for cutting DNA double strains after heterodimer forming were analysed.

关 键 词：锌指 切割酶 锌指核酸酶 显微注射 转基因动物 

分 类 号：S814[农业科学—畜牧学]