γ-聚谷氨酸温敏凝胶的合成及其生物相容性研究  

In vitro cell-biocompability study and synthesis of temperature-sensitive polyγ-glutamic hydrogel

作　　者：郭义兵[1] 石璐[1] 王凤志[1] 张鲁杰[1] 杨宁[1] 陈莉[1] 

机构地区：[1]天津工业大学材料科学与工程学院,天津300387

出　　处：《功能材料》2014年第14期14047-14051,共5页Journal of Functional Materials

基　　金：国家自然科学基金资助项目(31200719;50973084)

摘　　要：通过自由基共聚合方法成功制备了γ-聚谷氨酸温敏凝胶P(γ-PGA-co-NIPAAm),研究发现r=0.05、0.10和PNIPAAm凝胶分别在33.1,33.6和32.5℃处发生可逆的温度相转变行为。与PNIPAAm凝胶相比,共聚凝胶更利于L929的细胞粘附和增殖,表明引入聚谷氨酸利于L929细胞的生长。通过控制温度变化,细胞从凝胶表面自动脱附,其活性大于PNIPAAm凝胶表面自动脱附和从TCPS板上通过酶解法得到的细胞的生物活性。A novel thermo-responsive and biocompatible copolymer hydrogel based on N-isopropylacrylamide （NIPAAm）and polyγ-glutamic-based macromonomer （γ-PGA-AGE）was synthesized successfully by the free radical copolymerization.The copolymer hydrogel with r=0.05,r=0.10 and PNIPAAm hydrogel exhibits re-versible temperature-response at about 33.1,33.6 and 32 ℃ in water.Compared with conventional PNIPAAm hydrogel,the proliferation of L929 cells were higher on surfaces of r=0.05 copolymerized hydrogels,sugges-ting that the incorporation ofγ-PGA could stimulate cell growth.In addition,L929 cells could spontaneously detach from the surface of the poly（γ-PGA-co-NIPAAm）hydrogels without treating with any enzymes,and the detached cells at 20 ℃ showed higher viability than those detached from that on PNIPAAm hydrogel and TCPS.

关 键 词：凝胶 聚谷氨酸 N-异丙基丙烯酰胺 温度响应性 

分 类 号：R318.08[医药卫生—生物医学工程]