蟾酥主要活性成分对EV71-EGFP病毒感染性克隆的抑制作用  

The inhibitory effect of the main active ingredients of arenobufagin on EV71-EGFP virus infections clone

作　　者：陈嘉雯[1] 徐琳[1] 郜尧正 苏维恒[1,2] 陈维金[3] 

机构地区：[1]吉林大学生命科学学院,长春130012 [2]吉林大学生命科学学院艾滋病疫苗国家工程实验室,长春130012 [3]长春生物制品研究所有限责任公司,长春130062

出　　处：《中国新药杂志》2014年第16期1963-1966,共4页Chinese Journal of New Drugs

基　　金：吉林省科技发展计划青年基金(20140520001JH)

摘　　要：目的:研究蟾酥两个主要活性成分对EV71-EGFP病毒感染性克隆的影响,筛选出能抑制EV71-EGFP病毒感染性克隆的天然产物,为治疗由EV71引发的手足口病提供候选预防、治疗药物。方法:分别将含有华蟾酥毒基和酯蟾毒配基的细胞培养液加入到培养RDS细胞的96孔板中,同时加入EV71-EGFP病毒感染性克隆感染。15 h后于倒置显微镜下观察细胞生长状态、荧光显微镜下观察荧光值,并通过多标记微孔板检测系统对EGFP值进行定量研究。结果:与细胞对照组相比,添加蟾酥的两种活性成分虽能减少由EV71-EGFP病毒感染所造成的细胞病变,但仍有部分细胞形态发生变化;能提高细胞的密度和活力;能有效地减少细胞内EV71-EGFP感染性克隆的数量。结论:蟾酥的主要活性成分对EV71-EGFP病毒感染性克隆有一定的抑制作用。Objective： To study the inhibitory effect of the two main active ingredients of arenobufagin on EV71-EGFP virus infections clone to choose natural products that can inhibit EV71-EGFP infection and provide candidate drugs for prevention and treatment of hand-foot-mouth disease caused by EV71. Methods： RDS cells and the two main active ingredients from arenobufagin, Cinobufagin and Resibufogenin, in different dilution multiples were plated in 96-well plates at the same time, and EV71-EGFP was used to infect the cells. After 15 h, the plate were observed under inverted microscope for morphology. The fluorescence was tested by fluorescence microscope, and then the EGFP values were measured by a rnultimode microplate reader. Results： Compared with the control cells, the CPE caused by EV71-EGFP virus infection was reduced in the cells added with the two active ingredients of arenobufagin, but there was still morphology change in part of the cells. The density and vitality of RDS cells were increased, and the number of EV71-EGFP virus infectious clone in RDS cell was reduced effectively by the two ingredients. Conclusion： The two main active ingredients of arenobufagin have inhibitory effect on EV71-EGFP virus infection.

关 键 词：EV71-EGFP病毒感染性克隆 RDS细胞 蟾酥 活性成分 

分 类 号：R961[医药卫生—药理学]