不同外植体处理条件对“青杞1号”初代培养过程中组培苗的影响  

The impact on different explant processing conditions to the tissue culture seedlings of Lycium qingqi1 during the primary culture

作　　者：安焕霞[1] 王占林[2] 陈伟 

机构地区：[1]青海大学,青海西宁810016 [2]青海大学农林科学院,青海西宁810016 [3]四川省合江县林业局,四川合江646200

出　　处：《青海大学学报（自然科学版）》2014年第4期6-10,共5页Journal of Qinghai University(Natural Science)

基　　金：国家星火计划重大项目(2012GA870001);青海省科技支撑计划项目(2012-N-137)

摘　　要：以"青杞1号"嫩枝为试验材料,通过在不同时间段采集外植体、控制升汞浓度和消毒时间,比较分析了枸杞组培苗的启动时间、污染率和启动率。结果表明:最有利于"青杞1号"初代培养过程中组培苗生长的外植体处理条件为:取材日期7月1日,升汞浓度1.0 mg/L,消毒时间10 min。This article compared and analyzed the length of budding, rate of tissue c uhure seedlings of Lycium qingqil, according to collect polluting rate and germination explants, control concentration of HgC12 and length of disinfection in different months of the second half of 2013. The result showed that Julyl^th as the time of choosing explant, 1.0 mg/L as the concentration of HgC12 and 10 min as the length of disinfection were the best processing combination.

关 键 词：“青杞1号” 采集日期 升汞浓度 消毒时间 启动率 

分 类 号：S567.19[农业科学—中草药栽培]