原花青素对急性脊髓损伤大鼠核因子-кB和白细胞介素-6表达的影响  

Effect of Proanthocyanidin on Expression of Nuclear Factor kappa B and Interleukin-6 in Rats after Spinal Cord Injury

作　　者：马延仃[1] 谢少华[1] 杨拯[1] 杨海燕[1] 李晓[1] 张艺[1] 梁羽[1] 郭秀娟[1] 张奇兰[1] 张晓[1] 

机构地区：[1]成都医学院,四川成都市610500

出　　处：《中国康复理论与实践》2014年第8期713-717,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：2013年国家级大学生创新实验计划项目(No.201313705002);成都医学院大学生创新实验计划项目(No.CXXS201307)

摘　　要：目的观察原花青素对脊髓损伤后大鼠核因子-кB(NF-кB)、白细胞介素-6(IL-6)表达变化的影响。方法 36只健康成年Sprague-Dawley大鼠均分为3组:原花青素治疗组(A组)、甲基泼尼松龙治疗组(B组)和对照组(C组)。采用Allen法复制大鼠T9急性脊髓损伤模型,于术后1 d、3 d和7 d对各组大鼠行后肢运动功能BBB评分和斜板试验;免疫组织化学染色检测脊髓NF-кB和IL-6的表达。结果术后3 d、7 d,A组、B组大鼠BBB评分和斜板试验成绩均优于C组(P<0.05)。A组术后各时间点NF-кB表达均明显低于C组(P<0.01),B组术后3 d、7 d的NF-кB表达强度明显低于C组(P<0.01)。术后各时间点,A、B两组IL-6表达均低于C组(P<0.05)。结论原花青素可抑制脊髓损伤后大鼠脊髓组织NF-кB、IL-6的表达,有效抑制炎症反应,从而促进大鼠后肢运动功能的恢复。Objective To observe the effect of proanthocyanidin on the expression of nuclear factor kappa B （NF-кB） and interleukin-6 （IL-6） in rats with spinal cord injury （SCI）. Methods 36 healthy adult Sprague-Dawley rats were randomly divided into proanthocyanidin treatment group （group A, n=12）, methylprednisolone （MP） treatment group （group B, n=12） and model control group （group C, n=12）. Al-len＆#39;s method was used to establish the model of acute spinal cord injury in T9. On the 1st, 3rd and 7th days after operation, 4 rats in each group were assessed with Basso-Beattie-Bresnahan （BBB） scale and Slanting Board Test （SBT）, and the expression of NF-кB and IL-6 of the spinal cord were detected with immunohistochemistry. Results The scores of BBB and SBT were better in groups A and B than in the group C 3 and 7 days after SCI （P〈0.05）. Compared with group C, the expression of NF-кB in group A decreased all the time after SCI （P〈0.01）, and 3 and 7 days after SCI in group B （P〈0.01）;while the expression of IL-6 decreased all the time after SCI in groups A and B （P〈0.05）. Conclusion Proanthocyanidin may inhibit the expression of NF-кB and IL-6 in spinal cord after SCI in order to reduce inflammation and improve the hindlimb motor function in rats after SCI.

关 键 词：脊髓损伤 原花青素 运动功能 核因子-КB 白细胞介素-6 大鼠 

分 类 号：R651.2[医药卫生—外科学]