大鼠脊髓损伤后P物质与神经源性肠道功能障碍的关系  

Substance P in Neurogenic Bowel Dysfunction after Spinal Cord Injury in Rats

作　　者：龙志华[1,2] 高飞[1,2] 张锋良[1,2] 岳军忠[1,2] 王磊[1,2] 王烨[1,2] 刘文国[1,2] 徐青[1,2] 

机构地区：[1]首都医科大学康复医学院,北京市100068 [2]中国康复研究中心北京博爱医院,北京市100068

出　　处：《中国康复理论与实践》2014年第8期718-722,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：中国康复研究中心青年基金项目(No.2009-Q4)

摘　　要：目的探讨脊髓损伤后结肠中P物质与神经源性肠道功能障碍的关系。方法 60只体质量(220±40)g的雄性SpragueDawley大鼠随机分为假手术组(n=20)、正常对照组(n=20)和模型组(n=20)。氯胺酮60 mg/kg腹腔注射麻醉大鼠,利用NYU脊髓打击器,以75 g·cm致伤力制作T10脊髓损伤模型,分别于造模后24 h、1周、3周和5周时切除大鼠结肠组织制作标本,检测肠道传输功能,采用ELISA方法测定血清中和组织中的P物质含量,实时荧光定量PCR和Western blotting法检测P物质mRNA和蛋白表达。结果模型组大鼠脊髓损伤后出现肠道传输功能下降,且于造模后3周时肠道传输达到最低值;造模后3周时模型组血清和组织中P物质含量与假手术组相比均降低,结肠组织中P物质的mRNA及蛋白表达水平也下调,与假手术组、正常对照组相比具有显著性差异,假手术组P物质的表达是模型组的(3.12±0.51)倍(P<0.05)。结论大鼠脊髓损伤后神经源性肠道功能障碍与结肠中P物质的表达降低有关。Objective To investigate the relationship between neurogenic bowel dysfunction （NBD） and substance P in rats suffering from spinal cord injury （SCI）. Methods 60 male Sprague-Dawley rats, weighted （220±40） g, were randomly divided into three groups：sham group （n=20）, normal group （n=20） and model group （n=20） and then were subdivided into subgroups of 24 h, 1 week, 3 weeks, and 5 weeks respectively after SCI. SCI model was established at thoracic 10 segment of rat with NYU impactor device. The colon tissue of the rats was resected and stored. Substance P in serum and tissue was measured by ELISA. The tissue was examined by real-time RT-PCR and Western blotting to analyze the expression of substance P. Results The colon intestinal transmission function decreased and delineated at minimum value at 3 weeks in the model group. There was statistical significance with respect to the content of substance P in serum and tis-sue between the sham group and model group at 3 weeks. The expression of substance P in the sham group was （3.12± 0.51） times of the model group （P〈0.05）. Conclusion Substance P may take part in NBD after SCI in rats.

关 键 词：脊髓损伤 P物质 神经源性肠道功能障碍 大鼠 

分 类 号：R651.2[医药卫生—外科学]