尾状山羊草α-醇溶蛋白基因的克隆和序列分析  

Cloning and Sequence Analysis of α- Gliadin Genes from Aegilops caudata

作　　者：薄存瑶[1] 杜旭烨[1] 刘国娟[1] 尹华燕 王宏伟[1] 李安飞[1] 孔令让[1] 

机构地区：[1]山东农业大学农学院/作物生物学国家重点实验室,山东泰安271018

出　　处：《山东农业科学》2014年第8期6-9,14,共5页Shandong Agricultural Sciences

基　　金：国家转基因专项"优质转基因小麦新品种培育(2011ZX08002004-003)"资助

摘　　要：本研究利用一对α-醇溶蛋白基因的特异引物,从小麦近缘植物尾状山羊草Y46中克隆获得5个α-醇溶蛋白基因,与NCBI已提交的α-醇溶蛋白序列进行多重比对分析发现,本研究获得的α-醇溶蛋白基因与已知的小麦及其近缘植物中的α-醇溶蛋白基因序列具有较高的相似性,其编码的氨基酸序列存在一定的多态性;在潜在的致敏性上,在这5个α-醇溶蛋白序列中未发现任何已知的与乳糜泻病相关的抗原表位,这在小麦及其近缘植物中较为罕见;进化分析表明,来自C染色体组中的α-醇溶蛋白与来自M和U染色体组的α-醇溶蛋白具有较近的亲缘关系。Fiveα-gliadin genes were cloned with a pair of specific primers from Aegilops caudata Y46 . Their sequences had higher similarity with those of the published α-gliadin genes of wheat and its kindred plants in NCBI , and their encoded amino acid sequences had polymorphism .None of the published epitopes related with celiac disease were discovered in the five α-gliadins , which was rare in wheat and its kindred plants .The evolution analysis declared that the α-gliadins from C genome had a close relationship with those from M and U genomes .

关 键 词：尾状山羊草 α-醇溶蛋白 基因克隆 

分 类 号：S512.19[农业科学—作物学] Q785[生物学—分子生物学]