不同炮制方法对巴戟天中寡糖类成分和水晶兰苷含量的影响  

Effects of Different Processing Methods on Concents of Oligosaccharides and Monotropein in Morindae Officinalis Radix

作　　者：景海漪[1] 史辑[1] 崔妮[1] 贾天柱[1,2] 

机构地区：[1]辽宁中医药大学药学院,辽宁大连116600 [2]国家中医药管理局炮制原理解析重点研究室辽宁省中药炮制工程技术研究中心,辽宁大连116600

出　　处：《中国实验方剂学杂志》2014年第17期20-23,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年基金项目(81001635);国家发改委行业专项(201107007)

摘　　要：目的：考察不同炮制工艺对巴戟天中寡糖类成分和水晶兰苷含量的影响。方法：采用不同方法制备巴戟天炮制品,比较不同炮制品中寡糖类成分和水晶兰苷的含量。利用HPLC-CAD测定寡糖类成分含量,Shodex Asahipak NH2色谱柱,流动相乙腈（A）-水（B）梯度洗脱（0～15 min,86%～81%A;15～20 min,81%～75%A;20～35 min,75%～70%A;35～50 min,70%～58%A;50～60 min,58%～40%A;60～65 min,40%～20%A）;运用HPLC-DAD测定水晶兰苷含量,Venusil MP C18色谱柱,检测波长235 nm,流动相甲醇-0.4%磷酸溶液梯度洗脱（5∶95～28.8∶71.2,15 min）。结果：巴戟天不同炮制品中寡糖类成分总质量分数顺序为盐巴戟天〉巴戟肉〉制巴戟天〉生巴戟天;水晶兰苷含量排序为盐巴戟天〉生巴戟天〉制巴戟天〉巴戟肉。结论：巴戟天不同炮制品中寡糖类成分和水晶兰苷的含量差异较大,为巴戟天的饮片质量控制及炮制机制解析提供参考。Objective： To investigate effects of different processing methods on concents of oligosaccharides and monotropein in Morindae Officinalis Radix. Method： Processed products of Morindae Officinalis Radix were prepared by different methods,contents of oligosaccharides and monotropein in different processed products were compared. The content of oligosaccharides was determined by HPLC-CAD with Shodex Asahipak NH2 column,mobile phase of acetonitrile（A）-water（B） for gradient elution（0-15 min,86%-81%A; 15-20 min,81%-75% A; 20-35 min,75%-70% A; 35-50 min,70%-58% A; 50-60 min,58%-40% A;60-65 min,40%-20% A）; the content of monotropein was determined by HPLC-DAD with Venusil MP C18 column,mobile phase of methanol-0. 4% phosphoric acid for gradient elution（5 ∶ 95-28. 8 ∶ 71. 2,15 min） and detection wavelength at 235 nm. Result： The content of oligosaccharides was in the order of Morindae Officinalis Radix steamed by salt 〉Morindae Officinalis Radix without the wood〉 Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma 〉crude Morindae Officinalis Radix; while according to the content of monotropein was Morindae Officinalis Radix steamed by salt crude Morindae Officinalis Radix Morindae Officinalis Radix processed with Glycyrrhizae Radix et Rhizoma Morindae Officinalis Radix without the wood. Conclusion：Processing could greatly change contents of oligosaccharides and monotropein in Morindae Officinalis Radix,which provided a reference for pieces quality control and processing mechanisms analysis of Morindae Officinalis Radix.

关 键 词：炮制工艺 巴戟天 寡糖类成分 水晶兰苷 

分 类 号：R283.3[医药卫生—中药学] R283.6[医药卫生—中医学]