正交试验优选续断的水提工艺  

Optimization of Water Extraction Technology of Dipsaci Radix by Orthogonal Design

作　　者：朱海琳[1] 程宁波[2] 孟兆青[2,3] 丁岗[1,2] 王振中[1,2] 萧伟[1,2] 

机构地区：[1]南京中医药大学,南京210046 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中国药科大学,南京210009

出　　处：《中国实验方剂学杂志》2014年第17期31-33,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：目的:优选续断的水回流提取工艺。方法:以川续断皂苷VI转移率为评价指标,采用L9(34)正交试验考察液料比、提取时间、提取次数对续断提取工艺的影响。利用HPLC测定川续断皂苷VI含量,流动相乙腈-水(30∶70),检测波长212 nm。结果:最佳提取工艺为加15,10倍量提取2次,每次2 h;平均转移率(94.83±0.31)%。结论:该工艺简单、稳定、提取率高,适用于工业化大生产。Objective： To optimize water-extraction process of asperosaponin VI from Dipsaci Radix.Method： With transfer rate of asperosaponin VI as index,orthogonal design was used to optimize extraction process by taking liquid-solid ratio,extracting time and times as factors. The content of asperosaponin VI was determined by HPLC,mobile phase was acetonitrile-water（30 ∶ 70） and detection wavelength set at 212 nm.Result： Optimum process conditions were as follows： extracted twice with 15,10 times the amount of water,2 h for per time. Under these conditions,transfer rate of asperosaponin VI was（94. 83 ± 0. 309） %. Conclusion：This optimized process was simple,stable and productive,which was suitable for industrial production of Dipsaci Radix.

关 键 词：续断 提取工艺 正交试验 川续断皂苷VI 

分 类 号：R283.6[医药卫生—中药学]