四妙勇安汤当药苷、马钱苷提取率的拆方分析  

Decomposition Analysis on Sweroside and Loganin Extraction Rate Changes in Simiao Yongan Tang

作　　者：季新宇[1] 王翀[1] 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《中国实验方剂学杂志》2014年第17期102-105,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81173520)

摘　　要：目的：研究四妙勇安汤不同配伍组合对当药苷、马钱苷提取率的影响。方法：将四妙勇安汤拆方后,采用HPLC法,SunFireTMC18色谱柱（4.6 mm×150 mm,5μm）,流动相0.1%磷酸水-乙腈梯度洗脱,流速1.0 mL·min^-1,检测波长250 nm,柱温35℃,分析当药苷、马钱苷含量并计算其提取率。结果：测定了原方及拆方后共8组样品中当药苷、马钱苷的含量并计算相应提取率,当药苷、马钱苷的回归方程分别为Y=1 179 101.85X-28 583.08（r=0.999 2）和Y=1 353 095.24X-4 113.75（r=0.999 1）;线性范围分别为0.191 2～1.338 4,0.026 4～0.184 8μg;平均加样回收率分别为101.19%（RSD 2.83%）,102.59%（RSD 2.30%）。结论：与单味金银花相比,其他拆方组当药苷和马钱苷提取率均有不同程度的提高,全方合煎后2种成分的提取率最高,进而佐证了配伍的优越性和科学性。Objective： This study was carried out to investigate the influence of different compatibility combinations of Simiao Yongan tang on the extraction efficiency of sweroside and loganin. Method： The extraction rates of sweroside and loganin were determined by HPLC,using a SunFireTMC18 analytical column（4. 6 mm × 150 mm,5 μm） in prescription dismantling of Simiao Yongan tang. The mobile phase was composed of aqueous phosphoric acid（0. 1%） and acetonitrile using a gradient elution. Analytes were performed at 35 ℃ with a flow rate of 1. 0 mL·min^-1and a UV detection at 250 nm. Result： The sweroside and loganin in eight groups from the original prescription and dismantlementwere detected and analyzed. The regression equations for sweroside and loganin were Y = 1 179 101. 85X- 28 583. 08（r = 0. 999 2） and Y = 1 353 095. 24X- 4 113. 75（r = 0. 999 1）,respectively. The liner ranges were 0. 191 2-1. 338 4 μg for sweroside and 0. 026 4-0. 184 8 μg for loganin. The average recoveries of sweroside and loganin were 101. 19%（RSD 2. 83%） and 102. 59%（RSD 2. 30%）,respectively. Conclusion： Compared with single Lonicerae Japonicae Flos,the extraction rates of sweroside and loganin were improved in other compatibilities of Simiao Yongan tang. The whole prescription was superior to the other groups,which indicated that compatibility was preponderant and reasonable.

关 键 词：四妙勇安汤 拆方 配伍 当药苷 马钱苷 提取率 

分 类 号：R284.1[医药卫生—中药学]