沙煲暗罗的化学成分(Ⅰ)  

Chemical Constituents from Polyalthia oblique(Ⅰ)

作　　者：汪刘凯[1] 吴寿远 郑彩娟[1] 宋小平[1] 陈光英[1] 李小宝[1] 陈晓红[1] 

机构地区：[1]海南师范大学省部共建-热带药用植物化学教育部重点实验室,化学与化工学院,海口571158

出　　处：《中国实验方剂学杂志》2014年第17期117-120,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(21166009,81160391,81360478);海南省自然科学基金项目(211015)

摘　　要：目的:研究沙煲暗罗的化学成分。方法:采用95%乙醇提取,硅胶柱层析,Sephadex LH-20及重结晶等方法从沙煲暗罗茎和叶中分离化学成分,并根据光谱数据和理化性质确定各化合物的结构。结果:分离获得了8个化合物,其中生物碱类化合物为isooncodine(1),pendulamine A(2),甾体类化合物为stigmasta-4-ene-3,6-dione(3),β-谷甾醇(4),豆甾醇(5),其他化合物为对苯二酚(6),香草醛(7),十八碳二烯酸(8)。结论:所有化合物均为首次从该属植物中分离得到,抗菌活性测试结果表明,化合物3对大肠埃希菌(Escherichia coli)有抑制作用,其最小抑菌浓度(MIC)值达到5 mg·L-1;抗肿瘤活性测试结果表明,生物碱类化合物1对小鼠黑色素瘤细胞(B16F10)和肺癌肿瘤细胞(A-549)具有一定抑制活性,半抑制率(IC50)分别为78.6,49.3 mg·L-1。Objective： This study was carried out to study the chemical constituents from of Polyalthia oblique. Method： Compounds from 95% ethanolic extract of P. oblique were isolated by column chromatography on silica gel,Sephadex LH-20,together with recrystallization,and their structures were identified by their physical characteristics and spectral features. Result： Eight compounds were isolated from P. oblique,and identified as alkaloids isooncodine（1）,pendulamine A（2）,three steroids stigmasta-4-ene-3,6-dione（3）,β-sitosterol（4）,stigmasterol（5）,and three other compounds including hydroquinone（6）,vanillin（7）,octadecadienoic acid（8）. Conclusion： All compounds were isolated from P. oblique for the first time. Compound 3 showed inhibition activity against Escherichia coli,with minimal inhibitory concentrations（MIC） value of 5 mg·L^-1. Compound 1showed weak inhibition activities against SPC-A-1 and A-549 tumor cells,with IC50 values of 78. 6,49. 3 mg·L^-1.

关 键 词：沙煲暗罗 化学成分 抗菌活性 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]