LKB-1表达差异细胞系中黄芩苷和顺铂联合用药研究  

Study on the Drug Combination of Cisplatin and Baicalin in LKB-1 Differential Expression Cell Lines

作　　者：王士群[1,2] 庞丽萍[3] 朱宇珍[2] 叶华[2] 郑学宝[2] 

机构地区：[1]广东医学院药理学教研室,广东湛江524023 [2]广东天然药物研究与开发重点实验室,广东湛江524023 [3]广东医学院附属医院消化内科,广东湛江524023

出　　处：《中国现代应用药学》2014年第8期907-911,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：国家自然科学基金面上项目(81173240)

摘　　要：黄芩苷是黄芩中提取的黄酮类天然成分,具有显著的抗炎、抗肿瘤、促代谢等生理活性。腺苷酸活化蛋白激酶(AMPK)是调控能源代谢的主要途径,由上游LKB-1或CaMKKβ蛋白激酶调控。最新研究发现黄芩苷对于LKB-1低表达的细胞系中可通过CaMKKβ途径激活AMPK。本研究通过等效线图解法和Chou-Talalay法定性及定量评估了黄芩苷和顺铂在LKB-1表达差异的细胞系的相互作用。细胞凋亡结果发现:相比LKB-1高表达的肿瘤细胞系HepG2,黄芩苷在LKB-1低表达细胞系A549能够增强顺铂对凋亡的诱导;相反,对于高表达LKB-1的正常细胞系LO2,黄芩苷起到保护作用。Baicalin, one of the major flavoniods in Scutellaria baicalensis, is a potent activity in anti-inflammatory, anti-tumor and promoting metabolism. AMP-activated protein kinase（AMPK） plays an important role in energy metabolism and regulated mainly by two upstream kinases, LKB-1 or CaMKK. Recent studies show that CaMKK 13 is involved in AMPK by baicalin in LKB-1 deficient cell lines. In this study, isobologram and Chou-Talalay was respectively applied to qualitative and quantitative assessment of the interaction of cisplatin and baicalin in LKB-1 deficient or sufficient cell lines. In the apoptosis results, it was found that compared with LKB-1 sufficient tumor cell lines, baicalin could enhance the rate of apoptosis induced by cisplatin in deficient cells. On the contrary, baicalin protected against the development of apoptosis in LO2 ceils, a LKB-1 sufficient liver cell lines.

关 键 词：黄芩苷 顺铂 LKB一1 等效线图解法 Chou-Talalay 

分 类 号：R965.2[医药卫生—药理学]