HPLC测定人血清中多索茶碱的浓度  

Determination of Doxofylline in Human Serum by HPLC

作　　者：王金明[1,2] 王石健[2] 张晓芬[2] 杨赛成[2] 王彬辉[2] 

机构地区：[1]浙江中医药大学,杭州310053 [2]台州市立医院药剂科,浙江台州318000

出　　处：《中国现代应用药学》2014年第8期988-991,共4页Chinese Journal of Modern Applied Pharmacy

基　　金：台州学院基金项目(2012PY45)

摘　　要：目的建立高效液相色谱测定多索茶碱血药浓度的方法。方法用乙醚提取血样，以卡马西平为内标进行检测。色谱柱采用Waters C18（3．9mm×150mm，5μm），流动相为甲醇-水（35：65），流速为0．6mL·min^-1，检测波长为273nm，柱温为30℃。结果多索茶碱血清浓度在0．7～28btg·mL^-1内线性关系良好，线性方程为Y=0．3549X＋0，0039（r=0．9997）方法回收率为100．4％，日内、日间RSD分别为1．10％～3．87％和2．11％～4．86％（n＝5）。结论该方法快速、简便、准确、安全，可用于临床多索荼碱血药浓度的测定。OBJECTIVE To establish an HPLC method for determination of doxofylline in human serum. METHODS Ethyl ether was used as the exacted solvent, with carbamazepine as the internal standard. The column was Waters-C18 （3.9 mm×150 mm, 5μm）. The mobile phase was methanol-water（35： 65） and the flow rate was 0.6 mL＇min-1. The detection wavelength was 273 nm. The column temperature was kept at 30 ℃. RESULTS It showed a good linear range of 0.7-28 μg·mL^-1 for doxofylline, the linear regression equation was Y：0.354 9X＋0.003 9（r=0.999 7）. The methodological recovery was 100.4%, the intra-day RSD and the inter-day RSD were 1.10%-3.87% and 2.11%-4.86%（n=5）. CONCLUSION The method is proved to be rapid, simple, accurate and safe. It is suitable for determination of concentration of doxofylline in clinic.

关 键 词：高效液相色谱法 多索茶碱 血药浓度 

分 类 号：R917.101[医药卫生—药物分析学]