葛根素对性激素及前列腺增生组织中雌激素受体表达的影响  

Effects of Puerarin on Sex Hormone and Estrogen Receptor in Prostatic Hyperplasia

作　　者：陈江林[1] 熊丽娇[2] 江丽霞[2] 曾靖[3] 

机构地区：[1]江西省赣州市,人民医院341000 [2]赣南医学院第一附属医院,江西赣州341000 [3]赣南医学院,江曲赣州341000

出　　处：《时珍国医国药》2014年第8期1820-1822,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81160399)

摘　　要：目的研究葛根素对性激素水平及前列腺增生组织中雌激素α受体的影响。方法取50只昆明小鼠,乙醚浅麻醉,常规消毒,摘除双侧睾丸,缝合创口。术后第3天,各组每天皮下注射丙酸睾酮5.0 mg·kg-1·d-1,连续12 d,制造小鼠前列腺增生模型。50只小鼠随机分为5组,正常对照组,前列腺增生模型组,5.0,10.0,20.0 mg·kg-1·d-1葛根素组,观察各种处理12 d后小鼠前列腺湿重、前列腺指数、性激素水平及前列腺组织中雌激素α受体表达的影响。结果与模型组比较,10.0,20.0 mg·kg-1·d-1葛根素能显著抑制丙酸睾丸酮所致小鼠前列腺增生;明显降低小鼠血清睾丸酮(T)和雌二醇(E2)及增生组织中雌激素受体的表达。结论葛根素对丙酸睾丸酮所致小鼠前列腺增生具有显著的拮抗作用;其作用机制可能一定程度上与降低小鼠血清T,E2,T/E2及抑制雌激素α受体的表达有关。Objective To study the effect of Puerarin adjusting sex hormone and estrogen receptor - α（ ER -α） in prostatic hyperplasia tissue. Methods Thirty - two benign prostatic hyperplasia mice were modeled by subcutaneous injecting testosterone pro- pionate for 12 consecutive days, and randomly grouped in five,including：the normal control group, the model group of prostatic hyperplasia,treating group with 5.0,10.0,20.0 mg·kg^-1· d^-1 of Puerarin. After sacrificed the animal, prostate glandular wet weights,and itg propotion of total body weight, sex hormone including serum testosterone（ T）, estrodiol（ E2 ）contents and ER -α expression were measured. Results 10.0,20.0 mg·kg^-1 · d^-1 of Puerariu can significantly inhibit testosterone propionate induced mouse benign prostatic hyperplasia,reduce the serum testosterone,estrodiol contents and the ratio of T/E2, and inhibit the expression of ER -α. Conclusion Puerarin have significant antagonistic effect on experimentally induced mouse benign prostatic hyperplasia by testosterone propionate, the mechanism which may related to the regulative impact on the balance of sex hormone and the expression of ER -α.

关 键 词：葛根素 前列腺增生 睾丸酮 雌激素 雌激素受体 

分 类 号：R285.5[医药卫生—中药学]