中药清毒栓对HeLa细胞MHC-Ⅰ抗原呈递通路相关内质网分子伴侣表达的影响  

Influences of Qingdu Suppository on expression of MHC-Ⅰantigen presentation associated endoplasmic reticulum molecular chaperones in HeLa

作　　者：曹颖[1] 金哲[2] 徐丁洁[1] 赵舒[1] 杜晨光[1] 董玉山[1] 

机构地区：[1]河北联合大学中医学院,河北唐山063000 [2]北京中医药大学东方医院,北京100078

出　　处：《时珍国医国药》2014年第8期1825-1827,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.30873278);河北联合大学博士科研启动项目(No.35755599)

摘　　要：目的探讨中药清毒栓治疗宫颈高危型HPV感染的分子机制。方法以HPV18阳性的宫颈癌细胞系HeLa为细胞模型,清毒栓水提醇沉液、β-榄香烯、干扰素α-2b进行干预;分别采用Western blot及Real-time PCR检测MHC-Ⅰ抗原呈递通路相关内质网分子伴侣CNX、CRT、BIP的蛋白及基因表达差异。结果与对照组相比,3种药物均可不同程度的上调内质网分子伴侣CRT与CNX的蛋白表达,但对CRT与CNX的基因表达无影响(P>0.05);干扰素α-2b可上调BIP的蛋白表达(P<0.05),对BIP的基因表达无影响(P>0.05);清毒栓及β-榄香烯可上调BIP的基因表达,但对其蛋白表达无影响(P>0.05)。结论中药清毒栓及其有效成分之一β-榄香烯可通过上调内质网分子伴侣CNX及CRT的蛋白表达,促进宫颈癌细胞系HeLa的抗原呈递。Objective To explore the mechanism of Qingdu Suppository （QDS） in the treatment of high risk HPV infection. Methods Human cervical cancer cell line HeLa （ HPV 18 positive） was cutured in vitro and treated by the extract of QDS, β - elemene and IFNα - 2b. The protein and gene expression of MHC - I antigen presentation associated endoplasmic reticulum molecular chaperons including CNX, CRT and BIP were detected by Western blot and Real -time PCR independently. Result- s These three agents increased the protein expression of CNX and CRT in different degrees, whereas no significant difference was observed in gene expression of the two molecules（ P〉0.05 ）. IFNα- 2b up- regulated the protein expression of BIP（ P〈0.05 ） but had no effect on its gene expression（ P〉0.05 ）. QDS and β-elemene increased the gene expression of BIP（P〈0.05 ） but had no effect on its protein expression（P〉0.05）. Conclusion QDS and β-elemene as one of effective constituents in QDS both promote antigen presentation through up - regulating the protein expression of CNX and CRT function as molecular chaperones. This should be the key site in mechanism of action.

关 键 词：清毒栓 Β-榄香烯 干扰素Α-2B 抗原呈递 内质网分子伴侣 

分 类 号：R285.5[医药卫生—中药学]