补肾益智方有效部位群对M146L细胞分泌β淀粉样蛋白的抑制研究  

Inhibitory Action of Bushen Yizhi Recipe's Effective Parts on Secretion of β-Amyloid Protein in M146L Cell

作　　者：杨从[1] 侯雪芹[1] 闫蓉[1] 刘四军[1] 苏如玉[1] 章时杰[1] 张玉[1] 何文青[1] 陈云波[1] 王奇[1] 

机构地区：[1]广州中医药大学临床药理研究所,广东广州510405

出　　处：《时珍国医国药》2014年第8期2010-2013,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81273817);高等学校博士学科点专项科研基金(No.20114425110007);广东省重大科技专项(No.2012A080202017);广东省科技计划项目(No.2010A030100009);广东省高等学校科技创新项目(No.2012KJCX0032)

摘　　要：目的探讨补肾益智方有效部位群对M146L细胞分泌的β淀粉样蛋白的影响,寻找潜在的具有抑制Aβ分泌的中药复方有效群。方法体外培养稳定转染人类阿尔茨海默病(AD)β淀粉样前体蛋白(beta-amyloid protein precursor,APP)基因及突变型早老素1(prsenilin-1,PS1)基因的中华仓鼠卵巢上皮细胞系(M146L),使之高效产生β淀粉样蛋白Aβ1-42,建立Aβ1-42过度表达的细胞模型。加入待筛选的补肾益智方有效提取部位群,用MTT法检测不同浓度的补肾益智方有效提取部位群(0,12.5,50μg/ml)对M146L细胞的毒性作用,应用Western blot免疫印迹方法分别检测突变型PS1M146L和野生型APP751双转染稳定表达CHO细胞系分泌的Aβ1-42的变化。结果不同浓度的补肾益智方有效部位群对M146L存活均无影响,不具有细胞毒作用。其中浓度为12.5μg/ml有效部位群F1和F2对M146L细胞分泌Aβ1-42有抑制作用。结论一定剂量的补肾益智方有效提取部位群对M146L细胞分泌的Aβ1-42有明显的抑制作用,其机制有待进一步研究。Objective To study the inhibition of amyloid 13 - protein production in M 146L cell by effective parts of Bu - Shen - Yi -Zhi formula. Methods Aβ1-42 overpressing cell model in vitro was established by culturing Chinese hamster ovary having added the effective parts of Bu - Shen - Yi - Zhi formula. The MTT assay was used to test cytotoxicity of effective parts of Bu - Shen - Yi - Zhi formula at different concentrations and Western blot was carried out to evaluate Aβ1-42 production. Results Effective parts of Bu - Shen - Yi - Zhi formula at different degrees showed no cytotoxicity on M146L cells. The Fl and F2 effective parts of Bu -Shen -Yi -Zhi formula at the concentration of 12.51μg/ml had significant inhibition in Aβ1-42 production. Conclusion The suitable degree of effective parts of Bu - Shen - Yi - Zhi formula could inhibit Aβ1-42 production and its mechanism needs further study.

关 键 词：补肾益智方有效部位群 阿尔茨海默病 M146L细胞 Β淀粉样蛋白 

分 类 号：R285.5[医药卫生—中药学]