生长调节物质对半夏人工种胚形成的影响  

The Effects of Plant Growth Regulators on Artificial Embryo Formation of Pinellia ternata

作　　者：申起飞[1,2] 张明生[1,2] 李立青[1] 刘贵贤[1] 

机构地区：[1]贵州大学生命科学学院,贵州贵阳550025 [2]贵州大学山地植物资源保护与种质创新省部共建教育部重点实验室,贵州贵阳550025

出　　处：《山地农业生物学报》2014年第3期33-36,共4页Journal of Mountain Agriculture and Biology

基　　金：贵州省中药现代化科技产业研究开发专项项目[黔科合中药字(2011)5065号];贵州省中药材现代产业技术体系建设项目(GZ-CYTX-02)

摘　　要：以半夏叶片为外植体,研究了生长调节物质6-BA(6-苄氨基腺嘌呤)、2,4-D(2,4-二氯苯氧乙酸)、α-NAA(α-萘乙酸)、IBA(吲哚丁酸)对半夏人工种胚诱导和增殖的影响。结果表明,6-BA和2,4-D有利于半夏叶片愈伤组织的诱导和增殖,适宜培养基为MS+6-BA 1.0 mg/L+2,4-D 2.0 mg/L;6-BA、IBA和NAA对半夏人工种胚的诱导和增殖效果较好,适宜培养基为MS+6-BA 1.0 mg/L+IBA 2.0 mg/L或MS+6-BA1.0 mg/L+NAA 2.0 mg/L。结果为对半夏人工种胚诱导及工厂化提供了试验依据。The effects of 6 - benzyl amino adenine （6 - BA）, 2,4 - dichlorobenzene oxygen ethanoic acid （2,4 -D）, alpha naphthyl acetic acid （ct -NAA） and indole butyric acid （IBA） on the induction and pro- liferation of artificial embryos of Pinellia ternata were investigated herein using the leaves as the explants. The results showed that 6 - BA and 2,4 - D were advantageous for callus induction and multiplication, and the best medium was MS ＋ 6 - BA 1.0 mg/L ＋ 2,4 - D 2.0 mg/L. The 6 - BA, IBA and NAA were suitable for the induction and proliferation of artificial embryos, and the appropriate medium was MS ＋ 6 - BA 1.0 mg/L ＋ IBA 2.0 mg/L, or MS ＋ 6 - BA 1.0 mg/L ＋ NAA 2.0 mg/L. The results may facilitate the production of artificial seeds for this medical plant.

关 键 词：半夏 愈伤组织 快繁 脱分化 

分 类 号：Q945.5[生物学—植物学]