单纯与联合灌注法构建猪支气管动脉立体标本  

Constructing Bronchial Arteries Stereoscopic Specimen of Pig by Independent and Combined Casting Methods

作　　者：韩志磊[1] 王晓亮[2] 李路[3] 闫振龙[2] 火静萍 王白雪[2] 史军红[2] 刘英[1] 何玉琴[2] 

机构地区：[1]甘肃农业大学动物医学院,兰州730070 [2]甘肃农业大学生命科学技术学院,兰州730070 [3]兰州大学第一医院老年病科,兰州730000

出　　处：《中国农业科学》2014年第16期3315-3322,共8页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31260587)

摘　　要：【目的】改进和优化ABS血管铸型技术。创建猪支气管动脉立体标本的铸型方法,并构建猪支气管动脉立体标本。为探明猪支气管动脉在肺内的分支与分布状态提供实验方法。【方法】用改进和优化ABS铸型技术,采用支气管动脉单纯铸型和支气管动脉与支气管树或肺动脉联合铸型的方法构建猪支气管动脉的立体标本。【结果】(1)创建了猪支气管动脉立体标本的铸型方法。1猪肺支气管动脉单纯标本制作的方法要点:先分别在胸主动脉近心端和远心端插管并结扎胸主动脉。同时,为了防止在灌入铸型剂时经食管和气管周围的血管网发生渗漏,分别在胸主动脉插管处相应的位置结扎食管前端和后端,并在气管开口处插入盲端套管并结扎。然后调整肺的位置,使其背上腹下地置于解剖盘中。经胸主动脉间接向支气管动脉中注入铸型剂。灌注时强压梯度灌入60 mL 10%和100-160 mL 15%的ABS铸型剂,当橡胶管中间部位膨大后停止灌注。在连续灌注结束后5 h内随时观察橡胶管膨大部位的大小,当压力减小时,立即灌入20%的ABS铸型剂,保证主动脉内的正压力。将灌注好的标本置于冷水中静态硬化3-4 d,再用盐酸密闭腐蚀10 d左右。然后用流水漂浮和加压冲洗,再通过摘除凝块、打枝疏密和断枝再植修整后就可以获得完整的铸型标本。2猪肺支气管动脉与支气管树联合铸型标本制作的方法要点:首先,同时插管并结扎胸主动脉、食管和气管。然后经胸主动脉间接灌注铸型剂。间隔1-2 h后再对气管树进行灌注。最后同步硬化、腐蚀、冲洗和修复支气管动脉与支气管树联合标本。3猪肺支气管动脉与肺动脉联合铸型标本制作的方法要点:支气管动脉与支气管树联合铸型的方法和支气管动脉与肺动脉联合铸型的方法相似,不同之处在于在将铸型剂注入支气管动脉的同时,前者是将铸型剂注入�【Objective】The objective of this experiment is to improve and optimize the ABS vassal casting technique, establish a casting method for a Stereoscopic specimen of pig bronchial arteries, cast a stereoscopic specimens of pig bronchial arteries, thus providing an experimental way for the study of the branching structure and distribution of pig bronchial arteries in lung.【Method】With independent and combined casting methods of bronchial arteries, independent and combined bronchial artery casting specimens were obtained through improving and optimizing the ABS casting method. 【Result】 A method was established for making casts of the stereoscopic structure of bronchia arteries of a pig. This study proposed the steps and methods for making casts of the porcine independent and combined bronchial arteries. The steps and methods for making independent casts of the bronchial arteries were as follows： Firstly, the thoracic aorta was ligated and intubated in the proximal and distal ends of the thoracic aorta. Meanwhile the front end and the rear end of the esophagus were ligated at the corresponding position of the thoracic aorta intubation and the trachea was inserted the blind end sleeve and ligated in order to prevent the leakage around the trachea and esophagus vascular network during the infusion. Secondly, 60 mL of 10% ABS and 100-160 mL of 15% ABS were forced into the aorta through the aorta intubations indirectly. Infusion should be stopped when the middle part of rubber tube dilated. When the dilated part becomes smaller, 20% ABS should be infused to ensure the normal pressure after the end of the continuous infusion 5 h later. Thirdly, these specimens were hardened in water for 3 to 4 d, and then corroded with hydrochloric acid for about 10 d. At last, the integral specimens were obtained though washing under the running water and high pressure, and trimming elaborately. The major points for making combined casting of bronchial artery and bronchial tree were as follows： Firstly, the thoracic aorta,

关 键 词：猪 支气管动脉 标本 铸型方法 

分 类 号：S858.28[农业科学—临床兽医学]