不同类型诱导子对欧洲花楸悬浮细胞次生代谢的影响  

Effects of Different Types of Elicitors on Secondary Metabolism of Sorbus aucuparia Cell Cultures

作　　者：莫歌 黄蕾[1] 康利平[1] 华国栋[2] 郭兰萍[1] 

机构地区：[1]道地药材国家重点实验室培育基地/中国中医科学院中药资源中心,北京100700 [2]北京中医药大学东方医院,北京100078

出　　处：《中药材》2014年第6期927-931,共5页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金(81130070;81072989);国家科技支撑项目(2012BAI29B02;2012BAI28B002)

摘　　要：目的:探究不同类型诱导子对欧洲花楸悬浮细胞(SACC)次生代谢的影响。方法:用酵母提取物(yeast extract,YE)、重金属(铜、铅、镧)和化感物质(藁本内酯、阿魏酸、正丁基苯酞)三类诱导子处理SACC,用GC-MS检测其次生代谢产物变化。结果:与CK相比,YE处理后产生联苯类,长链烷酸、甾体数量减少;重金属处理后,长链烷酸数量增加,甾体、三萜数量减少;化感物质处理后长链烷酸、甾体和三萜类数量均减少。结论:三类诱导子对SACC次生代谢的影响不同,而同类型诱导子各处理组的作用基本一致,暗示不同类型诱导子会刺激SACC启动不同的次生代谢产物途径。Objective ：To investigate the effects of different types of elicitors on the secondary metabolism of Sorbus aucuparia cell cultures（SACC）. Methods：Added three different types of elicitors [ yeast extract （YE）, heavy metal ions and allelochemicals ] to the SACC,and detected the changes in the secondary metabolism of SACC using GC-MS. Results：Treatment of SACC with yeast extract in- duced the formation of biphenyls, and some long-chain fatty acids and steroids disappeared;The addition of heavy metal ions induced the formation of long-chain fatty acids, and some steroids and triterpenes disappeared ; And the addition of allelochemicals led to the disap- pearance of some long-chain fatty acids, steroids and triterpenes. Conclusion：These three types of elicitors have different effects on the secondary metabolism of SACC,but different elicitors of the same type act almost the same, which implies different kinds of elicitors would activate different pathways of secondary metabolism in SACC.

关 键 词：欧洲花楸 诱导子 次生代谢 

分 类 号：R282.2[医药卫生—中药学]