15种常见冬青属药用植物的分子鉴别  

Molecular Identification of 15 Species of Ilex Genus Based on ITS Sequence Analysis

作　　者：易帆[1] 韩凤鸣[1] 彭勇[1] 

机构地区：[1]中国医学科学院北京协和医学院药用植物研究所,北京100193

出　　处：《中药材》2014年第6期974-976,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:建立ITS区段的冬青属植物分子鉴别方法。方法:以15种冬青属植物为材料,采用离心柱型试剂盒提取总DNA,采用一对通用引物对ITS区段进行PCR扩增和测序;利用MEGA5.0软件分析,寻找差异位点,构建系统发育树。结果:测序后得到15种冬青属植物ITS比对序列为759 bp,种间存在216个差异位点。结论:ITS区段可以用于鉴别冬青属植物。Objective：To establish molecular method for identification of llex plants using the internal transcribed spacer（ITS） bar- code. Methods：DNA was extracted using centrifugal columnar kits. The ITS sequences of all experimental samples were amplified and sequenced using a pair of universal primers. The alignment and the phylogenetic tree were performed in MEGA5.0. Resuhs：The length of ITS sequence in each species was 759 bp,in which there were 216 sites discrepancy. Conclusion：DNA barcoding technology based on ITS sequence can be used to identify plants of llex genus.

关 键 词：冬青属 ITS 序列分析 

分 类 号：R282.5[医药卫生—中药学]