逍遥散有效部位对A375与HacaT细胞共培养模型TYR及其相关蛋白mRNA表达的影响  

Effects of Xiaoyao Powder Extract in Co-culture Model of Melanoma Cells and Keratinocytes on Expression of Tyrosinase and Its Related Protein

作　　者：张宁[1] 刘慧[1] 张明磊[1] 刘斌[1] 任燕冬[1] 王雪[1] 李建民[1] 

机构地区：[1]黑龙江中医药大学,黑龙江佳木斯154007

出　　处：《中药材》2014年第6期1025-1029,共5页Journal of Chinese Medicinal Materials

基　　金：教育部"春晖计划"合作科研项目(Z2010023)

摘　　要：目的:探讨逍遥散有效部位对A375与HacaT细胞共培养模型黑素合成的影响及机理。方法:制备逍遥散AB-8大孔树脂柱层析富集物,将其作用于以1∶2的比例构建的A375细胞与HacaT细胞共培养模型,采用Hunt法检测黑素含量,RT-PCR法测定TYR、TRP-1、TRP-2 mRNA的表达。结果:逍遥散大孔树脂柱层析40%乙醇洗脱部位对细胞共培养模型作用显著,与阴性对照组比较,逍遥散4、2 g/L组黑素量分别下调82.23%、93.01%;TYR、TRP-1、TRP-2 mRNA表达量分别下调29.11%、23.78%、20.05%和25.13%、15.02%、11.64%(P<0.01)。结论:逍遥散有效部位提取物可能通过下调TYR、TRP-1和TRP-2 mRNA的表达而降低人黑素瘤细胞的酪氨酸酶活性和黑素合成量。Objective：To observe the effects and mechanism of Xiaoyao Powder extract on the content of melanin in co-culture model of melanoma cells and keratinocytes. Methods ： Eluting components of Xiaoyao Powder was collected by AB-8 macroporous resin column. Different concentration extracts of Xiaoyao Powder were added into the co-culture model of A375 melanoma cells and HaCat ke- ratinocytes. Hunt method was used to detected the content of melanin. RT-PCR assay was used to detect the effects of the extract of Xi- aoyao Powder on the TYR, TRP-1 and TRP-2 mRNA expression in A375 melanoma cells. Results：Compared with the control group, the extract of Xiaoyao Powder down-regulated content of melanin and mRNA expression of TYR, TRP-1 and TRP-2 in A375 melanoma ceils by 82. 23% ,93.01% and 29. 11% ,23.78% ,20. 05% ;25.13%, 15.02% , 11.64% , respectively（ P 〈 0. 01 ）. Conclusion：The extract of Xiaoyao Powder can decrease the melanogenesis by down-regulating the mRNA expression of tyrosinase and its related protein.

关 键 词：逍遥散 黄褐斑 TYR TRP-1 TRP-2 

分 类 号：R285.5[医药卫生—中药学]