高盐饮食对雄性大鼠腰椎松质骨的影响及其机理研究  

Effect and mechanism of high salt diet on lumbar spongy bone in male rats

作　　者：贾欢欢[1] 卢丽[2] 陈珺[2] 杨国柱[2] 陆幸妍[2] 吴玉娥[1] 张钰[1] 李青南[2] 

机构地区：[1]广东省实验动物重点实验室/广东省实验动物监测所,广东广州510663 [2]广东药学院生命科学与生物制药学院,广东广州510006

出　　处：《广东药学院学报》2014年第4期463-466,共4页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的研究高盐饮食对雄性大鼠腰椎松质骨的影响及其机理。方法选择5周龄SD雄性大鼠24只随机分为2组,分别饲喂不同钠盐比例的鼠粮,正常组含NaCl 2.6 g/kg,高盐饮食组含NaCl 20 g/kg,连续喂养60 d。实验结束,取第5腰椎骨(LV5)松质骨进行骨形态计量学分析。结果静态参数:高盐组腰椎骨骨小梁面积百分数、骨小梁厚度均低于正常组(P<0.05);而骨小梁分离度大于正常组(P<0.05);骨小梁数量两组差异无统计学意义。动态参数:高盐组腰椎骨荧光周长百分数显著低于正常组(P<0.01);而骨形成率(BFR/TV、BFR/BS)及成骨细胞数目也低于正常组(P<0.05);骨矿化沉积率、骨形成率BFR/BV及破骨细胞数目差异无统计学意义。结论高盐饮食可导致腰椎骨量减少,骨结构变差,其机理主要是高盐导致骨形成减少,而不影响骨吸收,最终使骨吸收大于骨形成。Objective To study the effect and mechanism of high salt diet on lumbar bone in male rats. Methods Twenty-four,5-week old,male SD rats were divided into 2 groups,including the normal group（NS,2.6 g/kg NaCl） and the high salt diet group（HS,20 g/kg NaCl）. After 60 days feed,the fifth lumbar vertebra（LV5） was taken for bone histomorphometry. Results Trabecular area percentage and bone trabecular thickness were significantly lower in the HS group when compared with those in the NS group. Trabecular separation was significantly increased in the HS group. However,no significant difference was found in bone trabecular number in two groups. Compared with the NS group,percent labeled perimeter,bone formation rate and the osteoblasts number were significantly lower in HS-treated rats. But, no significant difference was found in bone mineralization sedimentary rate, bone formation rate BFRS/BV and number of osteoclasts in two groups. Conclusion High salt diet can lead to reduced bone mass and bone structure variation,which mechanism may be related to the inhibition of bone formation,without affecting the bone absorption.

关 键 词：高盐饮食 第五腰椎骨 成骨细胞 破骨细胞 骨形态计量学 

分 类 号：R580[医药卫生—内分泌]