荧光定量PCR熔解曲线分析技术检测MSM HIV感染者T细胞TCR β链CDR3谱序初探  

Preliminary study on detection of TCR CDR3 spectrum of TCR β gene repertoire by using FQ-PCR melting cure in the MSM HIV infected people

作　　者：李志峰[1] 曾艺[2] 张宏军[3] 冷文杰 陈亮[3] 李琴[3] 

机构地区：[1]重庆市疾病预防控制中心,重庆400042 [2]重庆市渝中区疾病预防控制中心,重庆400010 [3]重庆市永川区疾病预防控制中心,重庆402160

出　　处：《中国卫生检验杂志》2014年第15期2138-2140,共3页Chinese Journal of Health Laboratory Technology

基　　金：重庆市自然科学基金项目(cstc2011jjA10099);重庆市卫生局重点课题(2011-1-094)

摘　　要：目的监测HIV感染者的外周血样本TCRβ链互补决定区3(CDR3)谱系变化,发现HIV特异性TCR TRBV家族。方法用本实验室已建立的"荧光定量PCR溶解曲线法监测HIV感染者TCR CDR3谱系漂移技术",对22名健康男性、19名MSM HIV感染者的外周血样本TCRβ链CDR3变化进行监测。结果 MSM HIV感染者的TRBV 5.2,TRBV7,TRBV9和TRBV20的单(寡)性表达频率要高于健康男性人群(P<0.05),MSM HIV感染者的TRBV亚家族的缺失率(4.25%)高于对照男性健康人群的TRBV亚家族的缺失率(0.52%)(P<0.05)。结论在国内首次利用"荧光定量PCR溶解曲线法"监测HIV感染者TCRβ链CDR3漂移变化,发现MSM HIV感染者的TCR BV家族存在单(寡)性增生和缺失现象,非正态分布现象增加,多样性减少。Objective To monitor the change of TCR β CDR3 spectratyping and find the special T cell receptor（TCR） TRBV family in peripheral blood samples of the HIV-infected people. Methods The changes of the TCR β CDR3 spectratyping were monitored in 19 MSM HIV-infected cases and 22 healthy males by the established method＂Fluorescent quantitative PCR melting curve analysis＂. Results The monoclonal or oligoclonal expression of TRBV 5. 2,TRBV7,TRBV9 and TRBV20 were more frequent in MSM HIV-infected population than those in healthy males（P〈0. 05）,and the deletion rate of TRBV sub-family in MSM HIV-infected population was higher than that in healthy males（P〈0. 05）. Conclusion Fluorescent quantitative PCR melting curve analysis was firstly used to monitor TCR β CDR3 spectratyping changes in HIV-infected individuals. It was found there were phenomenons of monoclonal（or oligoclonal） proliferation and deletion,abnormal distribution increase and diversity decline in the TCR BV family of MSM HIV infected population.

关 键 词：荧光定量PCR溶解曲线法 互补决定区3 谱系漂移 

分 类 号：R512.62[医药卫生—内科学]