星斑川鲽、大菱鲆及其杂交后代的线粒体DNA序列比较分析  

Comparative analysis of genetic variability of mtDNA sequence in Platichthys stellatus,Scophthalmus maximus and their hybrids

作　　者：肖永双[1,2] 肖志忠[1] 刘清华[1] 滕照军 徐世宏[1] 马道远[1] 李军[1] 

机构地区：[1]中国科学院海洋研究所,山东青岛266071 [2]中国科学院海洋研究所(南通)海洋科学与技术研究与发展中心,江苏南通226000 [3]日照市水产集团总公司,山东日照276805

出　　处：《海洋科学》2014年第6期5-9,共5页Marine Sciences

基　　金：鲆鲽类现代产业技术体系建设项目(Nycytx-50-03);山东省科技发展计划(2011GGF01035;2011GHY11516);山东省自主创新成果转化重大专项(2012ZHZX1C0802);青岛市科技发展计划项目(12-4-1-51-hy;12-1-4-8-(6)-jch);江苏省自然基金青年基金资助项目(BK2012222)

摘　　要：为了揭示星斑川鲽(Platichthys stellatus)♀×大菱鲆(Scophthalmus maximus)♂杂交后代的种质遗传属性,作者采用线粒体(mt)DNA COI基因片段和控制区(D-loop)序列对星斑川鲽、大菱鲆及两种间的杂交后代(星斑川鲽♀×大菱鲆♂)遗传特性进行研究。研究结果显示,基于mtDNA COI和D-loop序列结果显示星斑川鲽和大菱鲆的种间遗传距离分别为22.9%和41.2%,相似的研究结果显示星斑川鲽♀×大菱鲆♂杂交后代与大菱鲆的遗传距离分别为22.9%和41.2%,遗传差异极其显著。而基于mtDNA D-loop序列结果显示星斑川鲽♀×大菱鲆♂杂交后代与星斑川鲽的遗传距离仅为0.4%,并且在mtDNA COI基因片段上两者序列完全一致,杂交后代在线粒体DNA上呈现明显的偏母遗传。Partial COI gene fragments and D-loop sequences of mitochondrial DNA were used to estimate genetic variability inPlatichthys stellatus,Scophthalmus maximus and their hybrids （P. stellatus♀×S. maximus♂）. Based on the analyses of the mtDNA COI gene and D-loop fragments, the genetic distances （D） betweenP. stellatusand S. maximus were 22.9% and 41.2% respectively. Similar result was obtained between the hybrids （P. stellatus♀×S. maximus♂） andS. maximus, which showed significant differentiation between hybrids and male parent. A very low differentiation （D= 0.4%） was observed between the hybrid and female parent based on the mtDNA D-loop sequences, and no genetic differentiation was found between them based on the mtDNA COI gene sequences. According to the above results, the hybrids （P. stellatus♀×S. maximus♂） showed strict maternal inheritance in mtDNA genome.

关 键 词：星斑川鲽(Platichthys stellatus) 大菱鲆(Scophthalmus maximus) 杂交 

分 类 号：Q347[生物学—遗传学]