自体血栓和凝血酶致大鼠局灶性脑梗死模型的建立与应用  

Establishment of focal cerebral infarction model by autologous thromb and thrombin as well as application

作　　者：郝春华[1] 王维亭[1] 郑海娟[1] 赵专友[1] 

机构地区：[1]天津药物研究院释药技术与药代动力学国家重点实验室,天津300193

出　　处：《药物评价研究》2014年第4期300-304,共5页Drug Evaluation Research

摘　　要：目的建立自体血栓和凝血酶致大鼠局灶性脑梗死模型。方法采用经颈外动脉向颈内动脉注入自体血栓和凝血酶栓塞大鼠大脑中动脉的方法,建立局灶性脑梗死模型并观察重组葡激酶(rSak)对该模型的改善作用。结果注入10μL自体血栓和50 U凝血酶时大鼠脑血流量较基础值降低,出现严重脑梗死神经症状、较大脑梗死范围和明显病理学改变,为理想模型。rSak 150 kU/kg能提高脑血流量,减少脑神经缺陷评分,缩小脑梗死范围,减轻病理学改变。结论建立了经颈内动脉注入自体血栓和凝血酶致大鼠局灶性脑梗死模型,并用rSak验证了该模型的稳定性及可靠性。Objective To establish a focal cerebral infarction model by autologous thromb and thrombin. Methods The focal cerebral infarction model was made by infusion of autologous thromb and thrombin from the external carotid artery into the internal carotid artery, and the improvement of rSak to this model was observed. Results After injection of 10 μL autologous thromb and 50 U thrombin, there were reduced rat cerebral blood flow, severe neurological symptoms, cerebral infarction, and obvious pathological changes compared to normal rats, that was thought to be the ideal model, rSak 150 KU/kg could improve the cerebral blood flow, reduce brain neurological symptoms, cerebral infarction, and pathological changes. Conclusion A model focal cerebral infarction model is established by infusion of autologous thromb and thrombin from the external carotid artery into the internal carotid artery, and the stability and reliability of the improvement of this model is observed by rSak.

关 键 词：大鼠 自体血栓 凝血酶 脑梗死 重组葡激酶 

分 类 号：R965.1[医药卫生—药理学]