正交试验结合指纹图谱优选补骨脂-肉豆蔻药对醇提工艺  

Optimization of ethanol extraction process of Psoraleae Fructus-Myristicae Semen drug pair by orthogonal test combined with fingerprint

作　　者：杨晓旭[1] 高家荣[1,2] 韩燕全[1,2] 庄星星 魏良兵[2] 

机构地区：[1]安徽中医药大学药学院,安徽合肥230038 [2]安徽中医药大学第一附属医院,国家中医药管理局中药制剂三级实验室,安徽合肥230031

出　　处：《中草药》2014年第15期2178-2183,共6页Chinese Traditional and Herbal Drugs

基　　金：国家中医药重点学科临床中药学建设项目(国中医药人教发[2012]32号)

摘　　要：目的优选补骨脂-肉豆蔻药对醇提的最佳工艺条件。方法通过L9(34)正交试验设计,考察乙醇体积分数、乙醇用量、提取时间、提取次数对提取工艺的影响,以HPLC指纹图谱特征峰总面积与补骨脂素、异补骨脂素、去氢二异丁香酚的量以及干浸膏得率为综合评判指标。结果最佳醇提工艺为乙醇体积分数50%,乙醇6倍用量,每次提取2 h,提取3次。结论本法为补骨脂-肉豆蔻药对醇提工艺的确定提供了依据。Objective To optimize the ethanol extraction process of Psoraleae Fructus-Myristicae Semen（psoralen-nutmeg） drug pair. Methods Using L9（34） orthogonal design, the effects of ethanol concentration, ethanol amount, extraction time, and extraction times on the extraction process were investigated. The contents of psoralen, isopsoralen, and dehydrodiisoeugenol, dry extract yield, and total area of HPLC fingerprint characteristic peaks were used as comprehensive evaluation indexes. Results The optimum process conditions were as follows： 50% ethanol, six times of the ethanol volume, extracted for three times, each time for 2 h. Conclusion The method provides the basis for the determination of ethanol extraction process of Psoraleae Fructus-Myristicae Semen drug pair.

关 键 词：补骨脂-肉豆蔻药对 醇提工艺 正交试验 HPLC 指纹图谱 特征峰总面积 补骨脂素 异补骨脂素 去氢二异丁香酚 

分 类 号：R284.2[医药卫生—中药学]