中药总苷成分群差式蒽酮-糖脎定量测量方法的建立  

Establishment of quantitative determination methods of total glycoside groups composition in Chinese materia medica by differential anthrone-sugar hydrazone

作　　者：唐宇[1] 胡超[1] 廖琼[1] 邹欢[1] 刘文龙[1,2] 杨岩涛[1,2] 贺红[1,2] 贺福元[1,2,3,4] 

机构地区：[1]湖南中医药大学药学院药剂教研室,湖南长沙410208 [2]中药药性与药效国家中医药管理局重点实验室,湖南长沙410208 [3]湖南中医药大学现代中药制技术与评价实验室,湖南长沙410208 [4]湖南中医药大学中医药超分子机理与数理特征化实验室,湖南长沙410208

出　　处：《中草药》2014年第16期2333-2338,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81073142;81270055;81173558)

摘　　要：目的建立中药复方总苷类成分的定量测定方法——差式蒽酮-糖脎比色法。方法用蒽酮比色法测定中药总糖苷（包括糖类）的量,然后采用糖脎硫酸显色法测定还原性糖类的量,两者相减,即得中药总苷类成分的量。结果蒽酮比色法的反应在6 h内完成;测定波长为620 nm;线性范围在80~180μg/mL,R2=0.994;回收率为99.2%,RSD为1.35%。糖脎硫酸显色法的反应在4 h内完成;测定波长为412 nm;线性范围在2~12 mg/mL,R2=0.991;回收率为99.5%,RSD为1.98%。采用本法测得补阳还五汤、六味地黄丸、穿心莲片、黄芪注射剂、茯苓多糖口服液5种中成药总苷的量依次为15.1、26.0、6.68 mg/g及21.3、16.9 mg/mL。结论首次创立了中药复方中总苷类成分定量测定方法,通过蒽酮比色法与糖脎硫酸显色法差值测算建立起以糖苷键为特征的成分群分析方法。方法学证明本法有较好的精确度,能用于中药总苷成分群的定量测定。Objective To establish a method for the determination of total glycosides in Chinese materia medica （CMM）--differential anthrone-sugar hydrazone colorimetry. Methods The content of total glycosides （including sugar） in CMM was determined by anthrone colorimetry, and then sugar hydrazone-sulfuric acid colorimetric method was used to determine the content of reducing sugars. The content of total glycosides came from the two subtraction. Results Anthrone colorimetric reaction was completed within 6 h, detection wavelength was 620 nm, linear range was 80--180 p,g/mL, correlation coefficient R2 = 0.994, and recovery was 99.2%, RSD was 1.35%. Sugar hydrazone-sulfuric acid color reaction method was completed within 4 h, detection wavelength was 412 nm,linear range was 2--12 mg/mL, correlation coefficient R2 = 0.991, and recovery was 99.5%, RSD was 1.98%. The total glycosides contents in five Chinese patent medicines such as Buyang Huanwu Decoction, Liuwei Dihuang Pill, Andrographis Tablet, Astragalus Injection, and Pachymaran Oral Liquid were 15.1, 26.0, 6.68 mg/g, and 21.3, 16.9 mg/mL. Conclusion This method is firstly established for the quantitative determination of total glycosides in CMM, and the ingredient group analysis method is established by anthrone colorimetry and sugar hydrazone-sulfuric acid colorimetric method. The methods have better accuracy, and can be used for the determination of total glycosides ingredients group of CMM.

关 键 词：总苷成分群 差式蒽酮-糖脎比色法 蒽酮显色反应 糖脎反应 补阳还五汤 六味地黄丸 穿心莲片 黄芪注射剂 茯苓多糖口服液 

分 类 号：R286.02[医药卫生—中药学]