HPLC法测定六味木香胶囊中木香烃内酯和去氢木香内酯  

Determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules by HPLC

作　　者：王海宁[1] 

机构地区：[1]贵州省食品药品检验所,贵州贵阳550004

出　　处：《中草药》2014年第16期2349-2351,共3页Chinese Traditional and Herbal Drugs

摘　　要：目的建立以HPLC法测定六味木香胶囊中木香烃内酯和去氢木香内酯的方法。方法色谱柱为VP-ODS柱（250mm×4.6 mm,5μm）,流动相为甲醇-水（72∶28）,检测波长为225 nm,柱温为25℃,体积流量为1.0 mL/min。结果木香烃内酯、去氢木香内酯进样量分别在203.12～2 031.20、207.84～2 078.40 ng与峰面积积分值呈良好的线性关系（r=0.999 9）;平均回收率分别为101.1%、101.2%,RSD分别为0.48%、0.73%（n=6）。结论建立的方法简便、准确、重复性好、专属性强,可用于该制剂的质量控制和评价。Objective To establish an HPLC method for the determination of costunolide and dehydrocostuslactone in Liuwei Muxiang Capsules. Methods The determination was performed on VP-ODS （250 mm ×4.6 mm, 5 μm） with column temperature set at 25℃. The mobile phase consisted of methanol-0.4% phosphoric acid water （72 ： 28） at a flow rate of 1.0 mL/min. The detection wavelength was set at 225 nm. Results The linear range of costunolide and dehydrocostuslactone was 203.12--2 031.20 ng and 207.84--2 078.40 ng （r = 0.999 9）, with average recovery at 101.1% and 101.2%, RSD = 0.48% and 0.73% （n = 6）. Conclusion The method proved is simple, accurate, reproducible, and specific, and it can be used for the quality control and evaluation of Liuwei Muxiang Capsules.

关 键 词：六味木香胶囊 HPLC 木香烃内酯 去氢木香内酯 质量标准 

分 类 号：R286.02[医药卫生—中药学]