解磷芽孢杆菌YC4液体培养基及发酵条件的优化  

Optimizing liquid medium components and culture conditions for a phosphate-solubilizing Bacillus strain YC4

作　　者：杨铿[1] 梁晓华[1] 杨莺莺[1] 陈永青[1] 佘妙虹[1] 洪敏娜[1] 徐创文[1] 陈海谊[1] 

机构地区：[1]中国水产科学研究院南海水产研究所/农业部南海渔业资源开发利用重点实验室,广东广州510300

出　　处：《广东农业科学》2014年第16期106-111,共6页Guangdong Agricultural Sciences

基　　金：中央级公益性科研院所基本科研业务费专项(2013YD09);国家现代农业(虾)产业技术体系建设专项资金(CARS-47);国家公益性行业(农业)科研专项(201103034)

摘　　要：对从养殖池塘中筛选出的一株具有解磷特性的芽孢杆菌YC4进行液体培养基和发酵条件的优化实验.在摇床培养条件下,研究了各营养源和主要影响因子对菌株YC4生长和芽孢形成的影响,并建立了菌株的生长曲线.结果显示：解磷芽孢杆菌YC4最佳发酵培养基配方为糖蜜10‰、大豆蛋白粉5‰、酵母膏5‰、磷酸二氢钾5‰、硫酸镁1.5‰、氯化钠15‰、玉米浆5‰;最适发酵条件为温度30℃,初始pH值为7.0～7.5,接种量1％,摇床转速200r/min;最适收获时间为24 h.优化后总菌量达1.23×109 CFU/mL,芽孢产量达1.01×109 CFU/mL,明显高于基础培养基发酵结果.To optimize the liquid fermentation medium and conditions for a phosphate-decomposing Bacillus strain YC4 isolated from aquaculture ponds, nutrient sources and major influencing factors were experimented under shake flasks to obtain their effects on the cell growth and spore formation and the growth curve of the strain was established. The optimal medium is determined to be composed of 10‰ of molasses, 5‰ of soybean protein powder, 5‰ of yeast extract, 5‰ of KH2PO4, 1.5‰ of MgSO4,15‰ of NaCl and 5‰ of corn steep liquor, and the optimal fermentation condition is 30℃ with initial medimn pH of 7.0-7.5, inoculum size of 1%, and rotation speed of 200 r/min. The best ingathering time should be in 24 hours. Under the optimized fermentation medium and conditions, the biomass concentration reaches 1.23×10^9 CFU/mLand the production of spores is 1,01×10^9 CFU/mL, both of which are apparently higher than the control experiment.

关 键 词：解磷微生物 芽孢杆菌 培养基优化 发酵条件优化 

分 类 号：Q939.96[生物学—微生物学]