水稻广亲和基因S5n新功能标记的设计与验证  

Design and validation for a new functional marker of wide compatibility gene S5n in rice

作　　者：田孟祥 余本勋 何友勋 张时龙 王嵩 叶永印 

机构地区：[1]毕节市农业科学研究所,贵州毕节551700

出　　处：《广东农业科学》2014年第16期134-137,共4页Guangdong Agricultural Sciences

基　　金：贵州省科技厅重大专项([2012]6005);[2013]6023-3);毕节市科技计划项目([2012]34-3)

摘　　要：广亲和基因S5n可恢复籼粳杂种育性,是亚种间优势利用的重要资源。S5n已经克隆,分子标记技术成为了鉴定其是否存在的快速有效手段。根据S5n存在136 bp碱基的缺失,设计均可在聚丙烯酰胺凝和琼脂糖凝胶这两种介质上电泳检测的S5n基因功能标记InDel-S5n,其扩增片段大小为224 bp(基因S5n)或360 bp(基因S5i或S5j)。通过10个常规水稻品种及1个F2群体230单株对InDel-S5n进行验证,结果表明,所设计标记均能较好地在琼脂糖凝胶和聚丙烯酰胺凝胶中进行检测,且能准确地鉴定供试材料是否包含目的基因及基因类型(纯合或杂合型),可见该标记可以用于S5n基因资源筛选、分子标记辅助选择育种。The indica japonica hybrid sterility can be restored by wide compatibility gene S5^n, which becomes an importanl resource to the utilization of heterosis between rice subspecies indica and japonica. The S5^n gene has been cloned. Obviously, molecular marker technology has become a fast and eft）ctive means to identify the gene. According the 136 hp deletion in the DNA sequence of S5^n, a new functional marker InDel-S5^n was developed based on the agarose gel electrophoresis and polyacrylamide gel electrophoresis, which amplified 224 bp （gene S5^n） or 360 bp （gene S5^i or S5^n）. To validate the InDel-S5^n, 10 conventional rice varieties and an F2 population of 230 plants were used, the experimental results indicated that the design marker could be detected relatively well in an agarose gel and polyacrylamide gel, and could accurately identify whether the test material comprising the gene and genotype （holnozygous or heterozygous）. Therefore, the InDel-S5^n is practical to be used for S5^n gene screening, molecular marker assisted selection breeding.

关 键 词：水稻 广亲和基因 S5N 功能标记 分子标记辅助选择 

分 类 号：S511.035.3[农业科学—作物学]