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作 者:李丽[1] 赵玉华[1] 赵文艳[1] 刘继志[1] 穆大伟[1] 王旋[1] 李万哲[1] 赵晨琼[1] 张政龙[1] 王宏坤[1]
出 处:《哈尔滨体育学院学报》2014年第4期17-20,共4页Journal of Harbin Sport University
基 金:黑龙江省自然科学基金项目(项目编号:D201269)
摘 要:为探求CNTF(睫状神经营养因子)基因的A/G多态位点与我国优秀单板U型场地运动员运动能力(尤其是力量素质)的相关性。文章选择20名优秀单板U型场地运动员和115名普通无专业训练的大学生作为研究对象,采用PCR-RFLP实验法测试CNTF基因的A/G多态位点,并用基因平衡定律测试结果,用基因平衡定律(H-W)检验,组间基因型与等位基因分布采用卡方检验方法。结果显示:对照组等位基因频率为A=18.7%,G=81.3%,基因型频率为A/A=6.1%,A/G=25.2%,G/G=68.7%;实验组等位基因频率为A=17.5%,G=82.5%,基因型频为A/A=10%,A/G=15%,G/G=75%。说明CNTF三种基因型分布频率的趋势为GG型>AG型>AA型,等位基因无差异(P>0.05)。提示CNTF基因A/多态性不能作为中国技能主导类优秀滑雪运动员选材的指标之一,但GG基因型所占比例大。In order to seek the relativity between CNTF ( Ciliary Neurotrophic Factor } gene and our country outstanding athlete veneer U - shaped ground movement ( Especially the power quality). This research mainly uses the method of PCR - RFLP, which selects 20 outstanding athlete veneer U -shaped venue as experimental group and 115 ordinary undergraduates as control group. The test results will use Hardy -Weinberg H -W to ensure the demographic representation of testing groups and use Chi - Square test to ensure allele and genotype distribution. Results: This research shows that allele frequency is A = 18.7% , G = 81. 3% and genotype frequency is A/A = 6. 1% , A/G = 25.2% , G/G = 68.7% for control group and that allele frequency is A = 17.5% , G = 82.5% and genotype frequency is A/A = 10% , A/G = 15% , G/G =75% for experimental group. Conclusion: The result presents that three CNTF genotype frequency trend is GG 〉 AG 〉 AA, and significant difference in al- lele is found between the two groups ( P 〈 O. 05 ). We suggest taking CNTF gene could not allele as one of the important indicators for talent identification of Chinese skill - dominated skiing athletes , but GG genotype the proportion of big.
关 键 词:运动能力 关联性 CNTF基因 A G多态性 单板U型场地
分 类 号:G804[文化科学—运动人体科学]
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