盐度对天津厚蟹不同器官组织中Na^+/K^+-ATP酶活力的影响  

Influence of Salinity on Activity of Na^+/K^+-ATPase in Different Organs and Tissues of Mudflat Crab,Helice tientsinensis

作　　者：徐敬明[1] 

机构地区：[1]重庆文理学院林学与生命科学学院,重庆402168

出　　处：《水产科学》2014年第8期483-487,共5页Fisheries Science

基　　金：重庆文理学院引进人才专项(200803)

摘　　要：在20℃下研究了盐度0、10、20、30、40和50对天津厚蟹鳃、肝胰脏及肌肉Na+/K+-ATP酶活力的影响。试验结果表明,天津厚蟹鳃中Na+/K+-ATP酶的活力为最高,其次为肝胰脏,肌肉最低,其间显著差异(P<0.05)。在9d试验中,自然海水对照组(30)及40、50盐度组天津厚蟹鳃Na+/K+-ATP酶的活力一直较稳定,只是40、50盐度组的Na+/K+-ATP酶的活力略高于30盐度组,彼此间差异不显著(P>0.05);其他各组3h后(20盐度3h时除外),鳃Na+/K+-ATP酶的活力与试验开始时相比显著升高(P<0.05),盐度越低,升高越大,其间及与对照组相比差异显著(P<0.05)。第24h,0、10、20盐度组鳃Na+/K+-ATP酶的活力达到最高或次高后,趋于稳定,并一直维持在较高的水平。但肝胰脏和肌肉中Na+/K+-ATP酶的活力与试验开始时及对照组(30)相比,变化很小(P>0.05)。The activities of Na＋ /K＋-ATPase in gill ,hepatopancreas and muscle were determined in mud-flat crab Helice tientsinensis exposed to different salinities （0 ,10 ,20 ,30 ,40 and 50） for 9 days at water temperature of 20 ℃ .The results showed that the order of the activity of the Na ＋ /K＋-ATPase in differ-ent organs and tissues was ranged at the same salinity as the following ：gill〉 hepatopancreas〉 muscle , with significant difference （P〈0 .05） .The stable activity of the Na＋ /K＋-ATPase in gill was observed in 9 day salinity exposure in the control group （30） ,40 ,and 50 groups ,just the latter two being slightly higher than the former ,without significant difference （P〉0 .05） .There was significant higher activities of the Na＋ /K＋-ATPase in gills in other groups except 20 salinity group for 3 h salinity exposure than that at the beginning of the exposure （P〈0 .05） ,increase in the activity with increase in salinity ,with signifi-cant difference （P〈0 .05） from the control group .In the 24 h salinity exposure ,the maximal activity of the Na＋ /K＋-ATPase in gill was found in 0 ,10 and 20 groups ,followed by stable ,and then at a high lev-el .But the activity of Na＋ /K＋-A T Pase in hepatopancreas and muscle in the crab exposed to the salinity for 9 days was kept few change compared with the crab in the control group （P〉0 .05） .

关 键 词：天津厚蟹 盐度 鳃 肝胰脏 肌肉 NA^+ K^+-ATP酶 

分 类 号：S965.28[农业科学—水产养殖]