淇河鲫不同器官组织中抗氧化酶活性和丙二醛含量的比较  

Comparison of Antioxidant Enzyme Activity and Malondialdehyde Content in Different Tissues and Organs in Crucian Carp Carassius auratus in Qihe River

作　　者：赵燕静[1] 蒋昕彧[1] 吴凡[1] 武向敏[1] 江红霞[1] 孔祥会[1] 

机构地区：[1]河南师范大学水产学院,河南新乡453007

出　　处：《水产科学》2014年第8期488-492,共5页Fisheries Science

基　　金：河南省高校科技创新人才支持计划项目(2011HASTIT012);河南省水产学重点学科项目(2012)

摘　　要：通过测定淇河鲫不同器官组织(肝胰脏、肾脏、脾脏、鳃和肌肉)超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶活性和丙二醛含量,并进行比较分析,以了解淇河鲫不同器官组织的抗氧化防护能力。结果显示,淇河鲫不同器官组织抗氧化酶活性不同。不同组织器官超氧化物歧化酶的活性为:肝胰脏>肾脏>肌肉>鳃>脾脏,不同器官组织间存在显著性差异(P<0.05);过氧化氢酶活性为:肝胰脏>肾脏>鳃>肌肉>脾脏,不同器官组织间差异显著(P<0.05);谷胱甘肽过氧化物酶活性为:肝胰脏>肾脏>鳃>肌肉>脾脏,差异显著(P<0.05);丙二醛含量测定值为:肝胰脏>肾脏>鳃>肌肉>脾脏,肌肉和脾脏间无统计学差异(P>0.05),其他器官组织间具有显著性差异(P<0.05)。淇河鲫肝胰脏和肾脏的丙二醛含量较高,而抗氧化酶活性也相应较高;脾脏丙二醛含量较低,而抗氧化酶活性也较低,说明丙二醛积累可诱导抗氧化酶活性增加。鱼类不同器官组织抗氧化酶活性和丙二醛含量存在组织特异性,可能与器官组织执行的生理功能和代谢水平不同有关。T he activities of superoxide dismutase ,catalase and glutathione peroxidase ,and malondialde-hyde content were studied in different tissues and organs of crucian carp Carassius auratus in Qihe river to understand differences in antioxidant defense ability in different tissues and organs in the fish .The results showed that there were different antioxidant enzyme activities in various tissues and organs .The order of superoxide dismutase activities in different tissues and organs was arranged as ：hepatopancreas〉 kidney〉muscle〉gill〉 spleen ,with significant difference in various tissues and organs （P〈0 .05） .The order of catalase activities in different tissues and organs was described as ：hepatopancreas〉kidney〉gill〉muscle〉spleen ,with significant difference in different tissues and organs （P〈 0 .05） .The range of glutathione peroxidase activities in different tissues and organs was expressed as ：hepatopancreas 〉 kidney 〉 gill 〉muscle〉 spleen ,with significant difference in different tissues and organs （P〈 0 .05） .The measured malondialdehyde contents in different tissues and organs were found as the order ：hepatopancreas〉kidney〉gill〉 muscle〉 spleen ,without significant difference in muscle and spleen （ P〉 0 .05） ,and significant difference in the other tissues and organs （P〈 0 .05） .There were higher malondialdehyde contents and higher antioxidant enzyme activities in hepatopancreas and kidney . However , there were lower malondialdehyde content and antioxidant enzyme activity in spleen ,indicating that the accumulation of malondialdehyde induced the antioxidant enzyme activities .T he specificity of antioxidant enzyme activities and malondialdehyde contents in tissues and organs is involved in physiological function and metabolism level in different tissues and organs of crucian carp .

关 键 词：淇河鲫 超氧化物歧化酶 过氧化氢酶 谷胱甘肽过氧化物酶 丙二醛 

分 类 号：S965.117[农业科学—水产养殖]