盐度胁迫对红耳龟Na^+/K^+-ATP酶及消化酶活性的影响  

Effects of Salinity on Na^+/K^+-ATPase and Digestive Enzyme Activities of Turtle Trachemys scripta elegans

作　　者：张珂[1] 洪美玲[1] 史海涛[1,2] 崔雪芹[1] 江萌萌 林多丽 

机构地区：[1]海南师范大学生命科学学院,海南海口571158 [2]中国科学院成都生物研究所,四川成都610041

出　　处：《水产科学》2014年第8期520-524,共5页Fisheries Science

基　　金：国家自然科学基金资助项目(30910103916);教育部科技重点项目(211145);海南省自然科学基金资助项目(303148)

摘　　要：将体质量(125.60±19.84)g的红耳龟饲养在盐度为5、15、25、35和自来水的水泥池中,进行了90d的盐度对其胁迫试验。试验结果表明,除胃组织中的Na+/K+-ATP酶活性随盐度的升高而显著下降(P<0.05)外,红耳龟的肠、肝脏及肌肉组织中的Na+/K+-ATP酶活性均随时间的延长先升后降,在盐度为5时达到最大值;胃蛋白酶活性随盐度的升高呈先升后降的趋势,在盐度5时活性最高,盐度25时酶活性不及对照组的1/2;肝脏脂肪酶活性随盐度的升高呈现下降—升高—下降的趋势,且在盐度15时活性最高,肠脂肪酶和肠淀粉酶活性随着盐度的升高而下降。高盐(>15)显著抑制红耳龟消化酶和Na+/K+-ATP酶的活性,影响其生长存活,但低盐(5以下)环境在一定程度上激活红耳龟消化酶和Na+/K+-ATP酶的活性,可正常存活3个月以上。红耳龟有可能入侵到更多水域包括半咸水水域。Turtle Trachemys scripta elegans with initial body weight of （125 .60 ± 19 .84） g were reared in a concrete tank at a salinity of 5 ,15 ,25 ,and 35 and freshwater for 90 days .The Na＋ /K＋-ATPase activi-ties in intestine ,liver and muscle were found to be increased firstly and then decreased with elapse of salin-ity stress ,the maximum at a salinity of 5 .In the stomach ,however ,Na＋ /K＋-ATPase activity was weak-ened with increase in salinity significantly （P〈0 .05） .The pepsin activities were consistent with the chan-ges of ambient salinity and all increased firstly then decreased ,the maximum at a salinity of 5 ,and the he-patic lipase activity was decreased firstly and increased secondly ,and then decreased ,the maximum at a salinity of 15 .Intestinal lipase and amylase activities were weakened with increase in salinity .It is conclu-ded that high salinity （over 15） led to inhabit Na＋ /K＋-ATPase activity ,thus mortality of the turtle . However ,since low salinity （below 5）promoted Na＋ /K＋-ATPase activity ,and hence the turtle can sur-vive in low salinity （a salinity of below 5） waters more than 3 months .It is speculated that the turtle could invade in a wide range of waters ,even brackish water .

关 键 词：红耳龟 盐度胁迫 消化酶 NA^+ K^+-ATP酶 

分 类 号：S966.5[农业科学—水产养殖]