Rapid Molecular Identification of Tribolium destructor  

作　　者：Chengwei ZHANG Liuping XU Miao LU Xiaosong LIANG Jian LI 

机构地区：[1]Changzhou Entry-exit Inspection and Quarantine Bureau [2]Municipal Bureau of Landscaping of Xinbei District

出　　处：《Agricultural Biotechnology》2014年第4期49-52,共4页农业生物技术（英文版）

基　　金：Supported by Science and Technology Project of Jiangsu Entry and Exit Inspection and Quarantine Bureau(2010KJ06)

摘　　要：In this study, a rapid molecular identification method of Tribolium destructor was established with PCR and PCR-RFLP （polymerase chain reaction-restriction fragment length polymorphism） technology. According to the results, （ 1 ） with PCR method, specific primers were designed based on CO1 gene of T. destructor for PCR amplification, and electrophoresis detection confirmed that PCR method could be used to rapidly and accurately identify T. destructor; （2） with PCR-RFLP method, two pairs of degenerate primers were used to amplify CO1 gene of Tribolium species, PCR products were digested with HindIII and detected by electrophoresis, results indicated that PCR-RFLP method could also be used for rapid identification of T. destructor in quarantine practice.In this study, a rapid molecular identification method of Tribolium destructor was established with PCR and PCR-RFLP （polymerase chain reaction-restriction fragment length polymorphism） technology. According to the results, （ 1 ） with PCR method, specific primers were designed based on CO1 gene of T. destructor for PCR amplification, and electrophoresis detection confirmed that PCR method could be used to rapidly and accurately identify T. destructor; （2） with PCR-RFLP method, two pairs of degenerate primers were used to amplify CO1 gene of Tribolium species, PCR products were digested with HindIII and detected by electrophoresis, results indicated that PCR-RFLP method could also be used for rapid identification of T. destructor in quarantine practice.

关 键 词：Tribolium destructor PCR PCR-RFLP Molecular identification 

分 类 号：Q503[生物学—生物化学]