Identification of Biocontrol Strain HJX1 and Determination of Its Antifungal Spectrum  

作　　者：Zhao Gengfeng Qiu Yimin Zhang Yunxia Xiong Guoru 

机构地区：[1]College of Science,Hainan Medical College [2]Institute of Tropical Biotechnology,Chinese Academy of Tropical Agricultural Sciences

出　　处：《Plant Diseases and Pests》2014年第3期11-14,19,共5页植物病虫害研究（英文版）

基　　金：Supported by Culture Fund of Hainan Medical College for Scientific Researchin 2013(HY2013-22)

摘　　要：A biocontrol strain HJX1,which had good inhibitory effect against Fusarium oxysporum f. sp. cubense,was identified as Bacillus subtilis based on traditional morphology,16 S rRNA gene sequence and fatty acid profiles. The sequence of 16 S rRNA gene had 99% homology to B. subtilis according to the DNA fragment of 1 521 bp amplified with a pair of universal primers,P0 and P6,of bacteria. Confront culture with pathogenic fungi showed that this strain had good inhibitory effect against 10 pathogenic fungi.A biocontrol strain HJX1,which had good inhibitory effect against Fusarium oxysporum f. sp. cubense,was identified as Bacillus subtilis based on traditional morphology,16 S rRNA gene sequence and fatty acid profiles. The sequence of 16 S rRNA gene had 99% homology to B. subtilis according to the DNA fragment of 1 521 bp amplified with a pair of universal primers,P0 and P6,of bacteria. Confront culture with pathogenic fungi showed that this strain had good inhibitory effect against 10 pathogenic fungi.

关 键 词：Bacillus subtilis HJX1 Identification Fusarium oxysporum f.sp.cubense Biological control 

分 类 号：S476[农业科学—农业昆虫与害虫防治]