用高世代回交群体定位大豆荚粒性状的QTL及上位性分析  

QTL Mapping and Epistasis Analysis of Pods per Plant and Seeds per Plant with an Advanced Backcross Population

作　　者：毛彦芝[1,2,3] 蒋洪蔚[1,2] 刘春燕[2] 辛大伟[1] 车京玉[1,2,3] 胡国华[2] 陈庆山[1] 

机构地区：[1]东北农业大学农学院,黑龙江哈尔滨150030 [2]黑龙江省农垦科研育种中心,黑龙江哈尔滨150090 [3]黑龙江省农业科学院克山分院,黑龙江克山161606

出　　处：《大豆科学》2014年第4期467-472,共6页Soybean Science

基　　金：教育部新世纪优秀人才支撑计划(NECT-1207-01);黑龙江省自然科学基金重点项目(ZD201213);现代农业产业技术体系(CARS-04-02A);黑龙江省博士后基金(LBH-Z12035);中国博士后基金(2012M520030);黑龙江省高校长江后备支持计划项目(2014CJHB004)

摘　　要：为进一步利用野生大豆资源,挖掘大豆荚粒性状关键基因,以黑龙江省大面积种植推广的绥农14为轮回亲本,野生种ZYD00006为供体亲本进行多代回交和自交,得到157株BC3F3株系,对其单株荚数、单株粒数、分枝数、单株粒重和百粒重进行相关性分析,并利用基于混合线性模型的QTL network 2.0软件和遗传搭车原理的卡方测验两种方法进行QTL定位,同时采用QTL network进行了上位性分析。结果表明：单株荚数和单株粒数都与单株粒重、分枝数极显著正相关,与百粒重极显著负相关。用两种方法共检测到4个单株荚数QTL,分别定位在B2,D1a,G和N连锁群上;5个单株粒数QTL,分别定位在F,J,D2和K连锁群上,其中定位在F连锁群上的Satt425~Satt663用两种方法都可以检测到。单株荚数和单株粒数上位性分析均检测到3对互作位点。One hundred and fifty-seven BC3F3 plants were constructed with Suinong 14 as recurrent parent and wild soybean ZYD00006 as donor parent in order to utilize the wild soybean germplasm and find the key genes on pod and seed.Pods per plant,seeds number per plant,branches,seeds weight per plant,and 100-seed weight were investigated.Correlation among those traits were analyzed with SPSS software.QTL and epistasis effects for pods per plant and seeds per plant were test by QTL network software 2.0 and chi-square analysis based on genetic hitchhiking.The result showed that pods per plant and seeds per plant were very significantly positive correlated with seeds weight per plant and branches,very negative correlated with 100-seed weight.A total of 4 QTLs related with pods per plant were detected on B2,D1 a,G,and N linkage group with those two methods.Five QTLs related with seeds per plant were tested on F,J,D2,and K linkage group,and the interval Satt425-Satt663 on F linkage group could be detected by two methods.Three interaction locus were test for pods per plant and seeds per plant,respectively.

关 键 词：高世代回交群体 单株荚数 单株粒数 遗传搭车原理 QTL定位 

分 类 号：S565.1[农业科学—作物学]