浊点萃取-紫外分光光度法同时测定厚朴酚与和厚朴酚的含量  

Simultaneous determination of magnolol and honokiol by UV spectrophotometry after cloud point extraction

作　　者：刘超美[1] 仲淑贤[1] 杨利宁[2] 邬梦璐 叶建萍[1] 陈建荣[1,2] 

机构地区：[1]浙江师范大学化学与生命科学学院,浙江金华321004 [2]浙江师范大学地理与环境科学学院,浙江金华321004

出　　处：《浙江师范大学学报（自然科学版）》2014年第3期307-311,共5页Journal of Zhejiang Normal University:Natural Sciences

基　　金：国家自然科学基金资助项目(21275131);浙江省自然科学基金资助项目(202127)

摘　　要：建立了以Triton X-114为表面活性剂的浊点萃取-分光光度法同时测定厚朴酚与和厚朴酚含量的新方法，探讨了溶液的pH，Triton X-114浓度、平衡温度和平衡时间等因素对浊点萃取的影响．在最佳条件下，厚朴酚与和厚朴酚含量的线性方程分别为Y1=5．89×10^-4x-1．14×10^-3（相关系数为0．9921），Y2=5．52×10^-4x-1．14×10^-5（相关系数为0．9992）；检测限分别为2．5μg／L和1．0μg／L．该方法成功用于药材中厚朴酚与和厚朴酚含量的测定，回收率为98．4％-104．3％．A new method of cloud point extraction-UV spectrophotometry based on using Triton X-114 as an extractant was developed for simultaneous determination of magnolol and honokiol in traditional Chinese medic -inal material.The effects of the pH of solution , concentrations of surfactant Triton X-114, equilibrium temper-ature and time on the efficiency of cloud point extraction were systematically examined .Under the optimized conditions, the linear equations were Y1 =5.89 ×10^-4x-1.14×10^-3（R2 =0.992 1）, Y2 =5.52×10^-4x-1.14×10^-5（R2 =0.999 2）, the detection limits were 2.5 μg/L, 1.0 μg/L for magnolol and honokiol, re-spectively .This method was applied to the determination of honokiol and magnolol in traditional Chinese me -dicinal material with recoveries of 98.4%~104.3%.

关 键 词：浊点萃取 分光光度法 厚朴酚 和厚朴酚 

分 类 号：O657.7[理学—分析化学]