甘草流浸膏中甘草酸含量测定方法的建立  

Content Determination of Ammonium Glycyrrhizic Acid in Extractum Glycyrrhizae by HPLC

作　　者：张振巍[1] 张娜娜 李国庆[1] 石磊[1] 

机构地区：[1]解放军第155中心医院,河南开封475000 [2]开封市中医院

出　　处：《中国药师》2014年第8期1419-1421,共3页China Pharmacist

基　　金：济南军区后勤科研计划(编号:CJN10L067)

摘　　要：目的：建立HPLC色谱法测定甘草流浸膏中甘草酸铵的含量.方法：采用HPLC色谱法在Daminsil C18 （250 mm×4.6 mm,5μm）色谱柱上梯度洗脱分离,乙腈-0.1％磷酸为流动相,梯度洗脱,检测波长为250 nm,流速为1.0 ml·min-1,柱温为30℃.结果：甘草酸铵在0.0468～0.2340 mg· ml-1范围内线性关系良好,平均回收率为97.83％,RSD为0.39％（n=9）.结论：该方法准确、灵敏度高、重现性好、简便可行,可作为含有甘草流浸膏制剂的质量控制方法.Objective ： To establish an HPLC method to determine the content of ammonium glycyrrhizinate in extractum glycyrrhi- zae. Methods： Tile operating conditions were a Diamonsil Cls （250 mm × 4.6 mm ,5 μm ） column and acetonitrile（ A ）-0.1% acetic aeid （B） as the mobile phase with gradieat elution. The flow rate was 1.0 m · min-1. The deteetion wavelength was set at 250nm for glycyrrhizic acid. The column temperature was maintained at 30℃. Result, s： The content of glycyrrhizic acid was linear within the range of 0. 046 8-0. 234 0 mg · ml 1 , and the average recovery was 97.83% with RSD of 0.39% （n = 9）. Conclusion： The method is accurate and eonvenient with high sensitivity and good repeatability,and can be used in the quality contrul of extractum glycyrrhizae.

关 键 词：甘草流浸膏 甘草酸铵 高效液相色谱法 

分 类 号：R927.2[医药卫生—药学]