高效毛细管电泳法同时测定保和丸中橙皮苷与连翘苷的含量  

Simultaneous Content Determination of Hesperidin and Phillyrin in Bao-He-Wan by HPCE

作　　者：张超云[1] 郝鹏飞[1] 席先蓉[2] 

机构地区：[1]南阳理工学院,南阳473004 [2]贵阳中医学院,贵阳550002

出　　处：《世界科学技术-中医药现代化》2014年第7期1668-1671,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：南阳理工学院2012年度青年科研基金项目(NYLG2012016):基于高效毛细管电泳法的保和丸质量控制研究;负责人:张超云

摘　　要：目的：建立高效毛细管电泳法测定保和丸中橙皮苷与连翘苷含量的方法。方法：采用区带毛细管电泳法,电泳条件为：未涂层石英毛细管柱（75 cm×50μm）,运行缓冲液30 mmol·L-1硼砂溶液（含8%乙腈,pH9.64）,压力进样50 kPa×20 s,电压20 kV,柱温20℃,检测波长254 nm。两次进样间用0.1 mol·L-1氢氧化钠和缓冲液分别冲洗10 min。结果：样品中橙皮苷与连翘苷均得到了很好的分离,其中橙皮苷与连翘苷分别在0.10-2.40 mg·mL-1（r=0.999 4）,0.07-0.84 mg·mL-1（r=0.999 2）范围内呈良好线性关系,平均回收率分别为99.85%,99.16%,RSD分别为2.34%,2.78%。结论：该方法简便、灵敏,在一定程度上弥补了传统方法的不足,适用于保和丸中橙皮苷与连翘苷的含量测定。This study was aimed to establish a HPCE method for the content determination of hesperidin and phillyrin in Bao-He-W an （BHW）. Fused silica capillary （75 cm × 50 μm） was employed and 30 mmol·L-1 borax so-lution （8% acetonitrile, pH9.64） was served as the running buffer. Other conditions were as follows： electrokinetic injection was 50 kPa × 20 s; analytical voltage was 20 kV; temperature was 20℃; and detection wavelength was 254 nm. The silica capillary was flushed with 0.1 mol·L-1 sodium hydrate and the running buffer for 10 min before each injection, respectively. The results showed that the linearity of hesperidin was in the range of 0.10-2.40 mg·mL-1 （r=0.999 4）, the average recovery was 99.85% and RSD=2.34%. The phillyrin was in the range of 0.07-0.84 mg·mL-1 （r=0.999 2）, the average recovery was 99.16% and RSD=2.78%. It was concluded that the method was rapid and sensitive. It can be used for the quality control of content determination of hesperidin and phillyrin in BHW.

关 键 词：保和丸 高效毛细管电泳 橙皮苷 连翘苷 

分 类 号：R284.1[医药卫生—中药学]