DGGE分析HA-A/A-MCO污泥减量工艺的菌群结构  

Analyzing Bacterial Community Structure of HA-A/A-MCO Sludge Reduction Process by Denaturing Gradient Gel Electrophoresis

作　　者：何进朝[1] 左宁[1] 曹悦[1] 吉芳英[2] 

机构地区：[1]重庆交通大学重庆西南水运工程科学研究所,重庆400016 [2]重庆大学三峡库区生态环境教育部重点实验室,重庆400045

出　　处：《中国给水排水》2014年第17期32-36,共5页China Water & Wastewater

基　　金：重庆市教委科学技术研究项目(KJ130420);重庆市基础与前沿研究计划项目(cstc2013jcyj A20013)

摘　　要：采用PCR-DGGE指纹图谱技术对HA-A/A-MCO污泥减量工艺的菌群结构进行了分析。结果表明,HA-A/A-MCO反应器中厌氧池、缺氧池和多级串联好氧池的微生物菌群均呈高度多样性分布,且各反应池拥有各自多样稳定的优势菌群,多种优势功能菌群的共同作用促进系统发挥出稳定良好的脱氮除磷和污泥减量性能。菌种鉴定发现,不动杆菌属是系统维持良好除磷效果的基础,螺旋体菌属承担去除有机质的功能,芽孢杆菌属和俊片菌属等在细菌培养区大量繁殖,并作为原、后生动物生长区中高等微生物的食源被捕食,通过这种高等对低等微生物的捕食作用促进了污泥的减量化。The bacterial community structure of HA-A/A-MCO sludge reduction process was analyzed by PCR-DGGE fingerprinting. The results indicated that the microbial communities had highly di- verse distribution in all anaerobic tank, anoxic tank and MCO tank of the HA-A/A-MCO process, and each tank had its own diversified and stable dominant microorganisms, as so the combined action of many dominant microorganisms promoted the system to exert good and consistent functions of phosphorus and nitrogen removal and sludge reduction. The species identification found that Acinetobacter sp. provided the basis of maintaining a good phosphorus removal performance, and Leptospira sp. undertaken the func- tion of organic matter removal. Bacillus sp., Lampropedia sp. and other bacteria could propagate in bac- terial culture area and be preyed upon by higher microorganisms in protozoan and metazoan growing are- as. Such predation of lower microorganisms by higher microorganisms could promote sludge reduction of HA-A/A-MCO system.

关 键 词：HA-A A-MCO工艺 PCR-DGGE指纹图谱 脱氮除磷 污泥减量 菌群结构 微生物捕食 

分 类 号：X703[环境科学与工程—环境工程]